Polimorfismo genetico de Streptococcus mutans isolados da cavidade oral de individuos com lesões de carie coronaria e radicular

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Dental root caries has been considered a very significant oral health problem in elderly and adults. Studies have suggested a common etiology for root and coronal caries, involving mutans streptococci. The aim of this study was to evaluate the colonization profile and clonal distribution of Streptococcus mutans isolated from oral cavity, presenting caronal and root surface caries lesions. The isolation and biochemical identification of this species were carried out using saliva samples, dental plaque, and tissue from caries lesions. In order to confirm their molecular identity, S. mutans and Streptococcus sobrinus were submitted to the PCR method, using primers specific for portions of the glucosyltransferase genes (gOO and gttl, respectively). The AP-PCR method was used to detect the genetic polymorphism of these species. Among the isolated and identified species, S. mutans (59,2%) show the greatest isolation, significantly differing from the other species - S. sobrinus (15,3%), Streptococcus ferus (6,5%) and Streptococcus cricetus (5,9%). Results showed that the subjects harbored two to ten genotypes of S. mutans, randomly distributed in different sites. The AP-PCR typing was efficient in demonstrating the genetic variability of the S. mutans, and able to distinguish fermenting and non-fermenting bacteria of melibiose. Different clonal types detected in oral cavity presenting coronal and root surface caries lesions can show different phenotypic properties (Supported by FAPESP (Process nº:00103490-9; 00106171-1)

ASSUNTO(S)

odontologia caries dentarias

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