Development and evaluation of residual virulence of a mutant strain of Brucella Abortus / Desenvolvimento e avaliação da virulência residual de uma cepa mutante de Brucella abortus

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Brucella spp. is an intracellular facultative gram-negative bacteria which is pathogenic for many species of mammals, causing brucelosis, a worldwide spread zoonosis. Therefore the search for more efficient alternatives of control, as the development of new strains that can be tested as potential immunogens, is necessary. In this study, we knockouted virB10 from Brucella abortus S2308 strain, generating a mutant strain probably incapable to produce the corresponding native protein. The gene virB10 is part of an operon that codifies for type IV secretion system, which is essential for the intracellular survival and multiplication of the bacteria in host cells. The knockout was carried through by the construction of the suicidal plasmid pBlue: virB10: kan and eletroporation in eletrocompetent cells of B. abortus S2308, leading to the exchange of the wild gene for the interrupted gene, containing the gene of resistance to kanamycin, for double homologous recombination. BALB/c mice were inoculated with S19, RB-51, ΔvirB10 strains of B. abortus and S2308 wild strain. The evaluation of the persistence of the strains showed that virB10 is essential for the maintenance of the virulence. These results support other studies concerning the immunogenic potential of this mutant strain.

ASSUNTO(S)

brucella abortus virb10 gene brucelosis medicina veterinaria brucelose

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