Dna Probes
Mostrando 25-36 de 3739 artigos, teses e dissertações.
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25. Detecção da microdeleção 7q11.23 por MLPA® e estudo clínico dos pacientes com síndrome de Williams-Beuren / Detection of the microdeletion 7q11.23 by MLPA® and clinical study of patients with Williams-Beuren syndrome
INTRODUCTION: Williams-Beuren syndrome (WBS) is a genetic disorder caused by a microdeletion in 7q11.23 region. It is characterized by typical facial dysmorphisms, mental retardation, hipersociable behavior, congenital heart disease, mainly supravalvular aortic stenosis (SVAS), and other variable congenital malformations. METHODS: 65 patients (40 males, 25 f
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/05/2012
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26. Valor da captura híbrida para o papilomavírus humano (HPV) no seguimento de pacientes submetidos à conização do colo uterino devido a lesão intraepitelial de alto grau por cirurgia de alta frequência (CAF) / The value of the HPV DNA test on the follow-up of the patients treated for high grade cervical intraepithelial lesions by conization with large loop excision of the transformation zone (LLETZ)
INTRODUÇÃO: A lesão intraepitelial cervical de alto grau causada pelo HPV, precursora do câncer cervical, é facilmente diagnosticada e seu tratamento pode ser realizado de maneira ambulatorial, sem muitas complicações. Mesmo assim, o seguimento das pacientes tratadas deve ser feito de maneira criteriosa e sistemática para que a recorrência ou persis
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/05/2012
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27. Impacto da presenÃa de atrazina na comunidade bacteriana do solo / Impact of atrazine on bacteriological soil community
Chemical contamination removal in soil and water depends on microbiological community that is able to degrade these compounds. There is a great evolutionary interest on studying microorganisms that metabolize the xenobiotic ones, since they have relatively been seen as new in the last five decades. Little is known about structure variation of microbiological
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/02/2012
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28. Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean
Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underly
Genetics and Molecular Biology. Publicado em: 2012
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29. ANÁLISE DA ASSOCIAÇÃO DE ALELOS HLA DE CLASSE II (DRB1) COM A HANSENÍASE EM UMA POPULAÇÃO DE SÃO LUÍS/MA / ANALYSIS OF THE ASSOCIATION OF HLA CLASS II ALLELES (DRB1) WITH LEPROSY IN A POPULATION OF SÃO LUÍS/MA
A hanseníase é uma doença infecto-contagiosa, que se manifesta principalmente por sinais e sintomas dermatoneurológicos. Estudos epidemiológicos têm mostrado que a variabilidade da resposta clínica à infecção causada pelo M.leprae está associadas aos fatores genéticos do hospedeiro. O sistema HLA (Human Leucocitary Antigen Antígeno Leucocitário
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/12/2011
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30. Determinação da porcentagem de espermatozóides portadores de cromossomos X e Y no sêmen sexado mediante PCR em tempo real / Determination of the proportion of X- and Y- bearing spermatozoa in bovine sexed semen by real time PCR
There are different biotechnologies to apply to increase the animal production nowadays, reproduction techniques are including. The artificial insemination (AI), for example, is a technique very used during many years ago and it is used at extensive production. The aim of this technique is to increase the numbers of pregnancies with just one ejaculation what
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/09/2011
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31. Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation
Genetics and Molecular Biology. Publicado em: 15/12/2011
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32. Molecular detection of Schistosoma japonicum in infected snails and mouse faeces using a real-time PCR assay with FRET hybridisation probes
A real-time polymerase chain reaction (PCR) assay with fluorescence resonance energy transfer (FRET) hybridisation probes combined with melting curve analysis was developed to detect Schistosoma japonicum in experimentally infected snails and in faecal samples of infected mice. This procedure is based on melting curve analysis of a hybrid between an amplicon
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
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33. Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles
Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic be
Brazilian Journal of Microbiology. Publicado em: 2011-09
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34. Chromosome identification in the Andean common bean accession G19833 (Phaseolus vulgaris L., Fabaceae)
Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA) were used as probes. Comparison to the Mesoamerican accession BAT93 sho
Genetics and Molecular Biology. Publicado em: 29/07/2011
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35. Synthesis, absorption and fluorescence spectral characteristics of trinucleus dimethine cyanine dyes as fluorescent probes for DNA detection
Neste trabalho descreve-se a preparação de seis corantes cianina dimetina trinucleares com núcleos de piridina, obtidos a partir da condensação de iodeto de trimetilpiridínio com aldeídos aromáticos heterocíclicos. As propriedades de absorção e fluorescência dos corantes foram estudadas em solventes com polaridades diferentes. O deslocamento hips
Journal of the Brazilian Chemical Society. Publicado em: 2011-01
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36. Isolation and characterization of microsatellites from the tapeworm Echinococcus granulosus
The Echinococcus granulosus genome was searched for microsatellites using 8 different repeated oligonucleotides as probes (GT15, CT15, AT15, CG15, CAT10, CAA10, CGG10 and CATA10). Southern blot experiments revealed that DNA regions containing GT, CAA, CATA and CT repeats are the most frequent in the E. granulosus genome. AT and CG probes showed no hybridizat
Publicado em: 2011