Dna Probes
Mostrando 13-24 de 3739 artigos, teses e dissertações.
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13. In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validat
Braz. J. Microbiol.. Publicado em: 2016-12
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14. Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis
Abstract The objective of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the IL10, NOS2A, and ESR2 genes and chronic periodontitis (CP) and aggressive periodontitis (AgP). Three groups of patients underwent periodontal and radiographic evaluations: CP (n = 61), AgP (n = 50), and periodontally healthy (control
Braz. oral res.. Publicado em: 10/10/2016
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15. Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experi
Genet. Mol. Biol.. Publicado em: 24/11/2015
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16. Associação de alelos HLA e aborto espontâneo recorrente em uma população de São Luís/Maranhão, na região Nordeste do Brasil
OBJETIVO: Investigar a associação dos alelos HLA-A, -B e -DRB1 com a ocorrência de Aborto Espontâneo Recorrente.
MÉTODOS: Estudo caso-controle com 200 mulheres com idade entre 18 e 35 anos, sendo a amostra de conveniência com 100 mulheres que tiveram aborto espontâneo recorrente idiopático e 100
Rev. Bras. Ginecol. Obstet.. Publicado em: 2015-08
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17. Optimisation of a quantitative polymerase chain reaction-based strategy for the detection and quantification of human herpesvirus 6 DNA in patients undergoing allogeneic haematopoietic stem cell transplantation
Human herpesvirus 6 (HHV-6) may cause severe complications after haematopoietic stem cell transplantation (HSCT). Monitoring this virus and providing precise, rapid and early diagnosis of related clinical diseases, constitute essential measures to improve outcomes. A prospective survey on the incidence and clinical features of HHV-6 infections after HSCT has
Mem. Inst. Oswaldo Cruz. Publicado em: 02/06/2015
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18. Investigation of the Interaction of 2-(2'-Hydroxyphenyl)-benzoxazoles and their Derivatives with B-DNA by Docking and Molecular Dynamics
In this work we carried out a study covering conformational analysis, docking calculations and molecular dynamics (MD) simulations of six excited state intramolecular proton transfer (ESIPT)-fluorescent 2-(2'-hydroxyphenyl)-benzoxazoles, interacting with the Dickerson-Drew (d(CGCGAATTCGCG)2) dodecamer in B-DNA conformation. In the analysis of the molecular d
J. Braz. Chem. Soc.. Publicado em: 2015-03
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19. The lowest diploid number in Testudines: Banding patterns, telomeric and 45S rDNA FISH in Peltocephalus dumerilianus, 2n = 26 and FN = 52 (Pleurodira, Podocnemididae)
The karyotype of the big-headed Amazon River turtle, Peltocephalus dumerilianus, is characterized based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas, Pará State, Brazil (1°30' S, 56°34' W). Here we present the first results on GTG and CBG-banding patterns, Ag-NOR staining and FISH, with telomeric and 45S rDNA sequences as probes
Genet. Mol. Biol.. Publicado em: 2014
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20. Differential detection of transposable elements between Saccharum species
Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analy
Genet. Mol. Biol.. Publicado em: 16/08/2013
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21. Intra- and interspecific chromosome polymorphisms in cultivated Cichorium L. species (Asteraceae)
Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome stai
Genet. Mol. Biol.. Publicado em: 19/07/2013
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22. Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers
The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridiz
Genet. Mol. Biol.. Publicado em: 2013
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23. A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks
Mycoplasma gallisepticum (MS) and Mycoplasma synoviae (MS) are important avian pathogens and cause economic losses to the poultry industry. Molecular biology techniques are currently used for a rapid detection of these pathogens and the adoption of control measures of the diseases. The aim of this study was to develop and validate a technique for simultaneou
Braz. J. Microbiol.. Publicado em: 2013
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24. Citogenética comparativa em espécies de Hypostomus (Siluriformes, Loricariidae, Hypostominae) : contribuição da fração repetitiva do genoma para a diversidade cromossômica do grupo
Hypostomus is the widest genus of the armored catfishes in South America. This group presents a great diversity in color pattern and morphology, and shows not conservative chromosomal features. Different classes of repetitive DNAs have distinct evolutionary dynamics in the genome of eukaryotes. Therefore, analyses and distribution of these sequences in chrom
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/11/2012