Conformational Changes
Mostrando 25-36 de 1400 artigos, teses e dissertações.
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25. Interação de nanotubos de carbono com segmentos de DNA
Single- and double-stranded DNA (deoxyribonucleic acid) molecules can strongly bind to single-walled carbon nanotubes (SWNT) via non-covalent interactions. Under certain conditions, the DNA molecule spontaneously self-assembles into a helical wrapping around the tubular structure of the carbon nanotubes to form DNA/SWNT hybrids, which are both stable and sol
Publicado em: 2007
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26. Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction-single-strand conformation polymorphism and direct sequencing methods
Mutations in the rpoB locus confer conformational changes leading to defective binding of rifampin (RIF) to rpoB and consequently resistance in Mycobacterium tuberculosis. Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) was established as a rapid screening test for the detection of mutations in the rpoB gene, and direct sequencin
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-09
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27. Insights into the role of hydration in protein structure and stability obtained through hydrostatic pressure studies
A thorough understanding of protein structure and stability requires that we elucidate the molecular basis for the effects of both temperature and pressure on protein conformational transitions. While temperature effects are relatively well understood and the change in heat capacity upon unfolding has been reasonably well parameterized, the state of understa
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-08
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28. Viral membrane fusion: is glycoprotein G of rhabdoviruses a representative of a new class of viral fusion proteins?
Enveloped viruses always gain entry into the cytoplasm by fusion of their lipid envelope with a cell membrane. Some enveloped viruses fuse directly with the host cell plasma membrane after virus binding to the cell receptor. Other enveloped viruses enter the cells by the endocytic pathway, and fusion depends on the acidification of the endosomal compartment.
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-06
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29. Syindiotactic polypropylene/EPDM blends, influence of processing and of thermal history in the physical and mechanical properties / Blendas de polipropileno sindiotatico com EPDM : influencia do processamento e da historia termica nas propriedades fisicas e mecanicas
The sPP containing 30 % of racemic pentads is a semicrystalline polymer presenting different crystalline polymorphs which depend on the thermo-mechanical history. Under heating sPP can recrystallize in a more perfect and thermodynamically stable form. The polymorphs differ in the density and also in the lamella thickness. The insertion of defects in the crys
Publicado em: 2005
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30. Quenching of the intrinsic fluorescence of bovine serum albumin by chlorpromazine and hemin
The binding of chlorpromazine (CPZ) and hemin to bovine serum albumin was studied by the fluorescence quenching technique. CPZ is a widely used anti-psychotic drug that interacts with blood components, influences bioavailability, and affects function of several biomolecules. Hemin is an important ferric residue of hemoglobin that binds within the hydrophobic
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-07
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31. A INIBIÇÃO DA ENZIMA δ-AMINOLEVULINATO DESIDRATASE POR MONOSSACARÍDEOS NÃO É MEDIADA PELA OXIDAÇÃO DE GRUPOS -SH
Heme pathway enzyme δ-aminolevulinate dehydratase (δ-ALA-D) is a good marker for oxidative stress and metal intoxication. This sulfhydrylic enzyme is inhibited in several oxidative pathologies like lead, mercury and aluminum intoxication, selenium organic species exposition and diabetes. Oxidative stress is one of cause of diabetes complications. T
Publicado em: 2004
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32. A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-08
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33. Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence
Potato apyrase, a soluble ATP-diphosphohydrolase, was purified to homogeneity from several clonal varieties of Solanum tuberosum. Depending on the source of the enzyme, differences in kinetic and physicochemical properties have been described, which cannot be explained by the amino acid residues present in the active site. In order to understand the differen
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-07
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34. Correlación entre los defectos de aplomo y la cojera del caballo
One hundred and seventy horses (144 males and 26 females) were studied to assess the influence of defects on limb conformation on the development of lameness. Animals were used for sport or pleasure, and most of them (N= 108, 63,5%) were Andalusian horses. Horses with or without lameness were selected according to the presence of conformational defects on th
Publicado em: 2000
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35. Detecção de "upward-going" muons no experimento LVD
In this work we present a careful study about the potentiality of the small angle scattering technique and the inverse scattering problem ie., the reconstruction of the particle shape directly from the scattering profile. In this way, we made many simulations to test and analyzed various methods. First I show the general scattering theory, emphasizing the sc
Publicado em: 2000
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36. Estudo de difração de raios-x a alta resolução da beta-lactoglobulina bovina
Beta-lactoglobulin (BLG), a very abundant protein found in several species of mammals belongs to the lipocalin family. Under physiological conditions, BLG is a dimmer of 162 aminoacids per monomer, with a molecular mass of approximately 18kDa in each subunit. Although BLG has important physicochemical properties, its biological function remains unclear. BLG
Publicado em: 1999