Catabolite Repression
Mostrando 1-12 de 670 artigos, teses e dissertações.
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1. Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression
Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. T
Braz. J. Microbiol.. Publicado em: 2017-07
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2. Yeast biomass production: a new approach in glucose-limited feeding strategy
The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to met
Braz. J. Microbiol.. Publicado em: 2013
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3. Production and partial characterization of serine and metallo peptidases secreted by Aspergillus fumigatus Fresenius in submerged and solid state fermentatio
Enzyme production varies in different fermentation systems. Enzyme expression in different fermentation systems yields important information for improving our understanding of enzymatic production induction. Comparative studies between solid-state fermentation (SSF) using agro-industrial waste wheat bran and submerged fermentation (SmF) using synthetic media
Braz. J. Microbiol.. Publicado em: 2013
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4. Production of biodegradable plastics using sugarcane bagasse hemicellulosic hydrolysate. / Produção de plásticos biodegradáveis utilizando hidrolisado hemicelulósico de bagaço de cana-de-açúcar.
The aim of this thesis is to produce poly3-hydroxybutyrate (P3HB) and poli-3-hidroxibutirate-co-3-hydroxyvalerate (PHB-co-3HV), biodegradable polymers, using hemicellulosic hydrolysate, rich in xylose, from sugarcane bagasse. Metabolic flux analysis in silico of xylose metabolism indicated that, though metabolism redirection is possible to increase P3HB yiel
Publicado em: 2010
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5. Identificação, caracterização e análise da expressão de genes de resposta ao estresse oxidativo em Lactobacillus delbrueckii UFV H2b20 / Identification, characterization and expression analysis of oxidative stress response genes in Lactobacillus delbrueckii UFV H2b20
Oxidative stress is an important cause of viability loss in lactic acid bacteria used in food industry and as probiotics. This work aimed at identifying and characterize genes involved in the oxidative stress response in Lactobacillus delbrueckii UFV H2b20, a potencial probiotic strain. Therefore, dps, which encodes a ferritin-like protein, and pox, which en
Publicado em: 2008
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6. Impact of the reg1 mutation glycocen accumulation and glucose consumption rates in Saccharomyces cerevisiae cells based on a macrokinetic model
In S. cerevisiae, catabolite repression controls glycogen accumulation and glucose consumption. Glycogen is responsible for stress resistance, and its accumulation in derepression conditions results in a yeast with good quality. In yeast cells, catabolite repression also named glucose effect takes place at the transcriptional levels, decreasing enzyme respir
Brazilian Journal of Chemical Engineering. Publicado em: 2003-09
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7. Produção de B-Galactosidade por Erwinia aroidae cultivada em soro de queijo
Production of intra and extracellular /3-galactosidase by Erwinia aroidea grown in cheese whey was studied. Preliminary experiments with edenrneyers and desproteinized cheese whey showed the optimallactose concentration of 5.5% when the lactase activity achieved 434.10 VI/ml and cellular dried mass 1.9048 g/l for 12 hours culture. Optimals temperature and pH
Publicado em: 1995
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8. Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.
Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the
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9. Catabolite repression of the Bacillus subtilis hut operon requires a cis-acting site located downstream of the transcription initiation site.
Expression of the Bacillus subtilis hut operon is subject to regulation by catabolite repression. A set of hut-lacZ transcriptional fusions was constructed and used to identify two cis-acting sites involved in catabolite repression. The hutOCR1 operator site lies immediately downstream of the hut promoter and weakly regulates hut expression in response to ca
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10. Mutations in Catabolite Control Protein CcpA Separating Growth Effects from Catabolite Repression
Carbon catabolite repression in Bacillus megaterium is mediated by the transcriptional regulator CcpA. A chromosomal deletion of ccpA eliminates catabolite repression and reduces the growth rate on glucose. We describe four single-amino-acid mutations in CcpA which separate the growth effect from catabolite repression, suggesting distinct regulatory pathways
American Society for Microbiology.
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11. Determination of the cis sequence involved in catabolite repression of the Bacillus subtilis gnt operon; implication of a consensus sequence in catabolite repression in the genus Bacillus.
The mechanism underlying catabolite repression in Bacillus species remains unsolved. The gluconate (gnt) operon of Bacillus subtilis is one of the catabolic operons which is under catabolite repression. To identify the cis sequence involved in catabolite repression of the gnt operon, we performed deletion analysis of a DNA fragment carrying the gnt promoter
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12. Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae.
The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated without a glutamate supplement, had a dimin