Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.
AUTOR(ES)
Weickert, M J
RESUMO
Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many mutations reduced the repression ratio 3- to 11-fold, some also caused a 2-fold or greater increase in amylase production. Others caused hyperproduction without affecting catabolite repression. Homologous sequences in other catabolite-repressed B. subtilis promoters suggest a common regulatory site may be involved in catabolite repression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=54508Documentos Relacionados
- Oligonucleotide site-directed mutagenesis in Xenopus egg extracts.
- Improved site-directed mutagenesis method using PCR.
- Toward computer-aided site-directed mutagenesis of enzymes.
- Recent advances in gene mutagenesis by site-directed recombination.
- Improved method for PCR-mediated site-directed mutagenesis.