Assay
Mostrando 1-12 de 26456 artigos, teses e dissertações.
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1. Há algum significado clínico a presença de Anti-HCV positivo (ELISA) quando PCR para Hepatite C está negativo?
De acordo com revisão do Clinical Knowledge Summaries (1), na presença de um paciente com exame Anti-HCV (ELISA) positivo e PCR para Hepatite C negativo, sugere-se repetir o PCR em um intervalo de 6 meses, para confirmar a ausência de infecção pelo vírus da Hepatite C.
Em paciente com Anti-HCV positivo, após dois exames PCR negativos realizad
Núcleo de Telessaúde Rio Grande do Sul. Publicado em: 12/06/2023
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2. Com quanto tempo de evolução da doença, em caso de suspeita de dengue, está indicada a solicitação de sorologia?
A partir do sexto dia do início dos sintomas, faz-se a sorologia pelo Método Enzyme-linked Immunosorbent Assay ( Elisa); detecção de antígenos virais: NS1, isolamento viral, RT-PCR e imunohistoquímica. Devem ser solicitados até o quinto dia do início dos sintomas.
Se positivos confirmam o caso; se negativos, uma nova amostra para sorologia I
Núcleo de Telessaúde Sergipe. Publicado em: 12/06/2023
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3. Qual a conduta diante de um contato de tuberculose com resultado de teste tuberculinico acima de 10 mm?
Frente a um contato de tuberculose, com resultado do teste tuberculínico acima de 10mm, deve-se realizar o tratamento para tuberculose latente
. Segundo protocolos do Ministério da Saúde tem indicação de tratamento para infecção latente pelo Mycobacterium tuberculosis contatos adultos e crianças com PT ≥ 5mm ou Interferon Gamma Realease As
Núcleo de Telessaúde Maranhão HU-UFMA. Publicado em: 12/06/2023
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4. METABOLIC PROFILING AND CYTOTOXIC ACTIVITY OF METHANOL EXTRACTS FROM CHAMAECRISTA DUCKEANA (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE)
The genus Chamaecrista comprises more than 330 species, with only a few studies on their chemical composition and biologic activities. In this study, the phytochemical profile of leaf, stems, and fruits extracts of the C. duckeana were examined by UPLC ESI HRMS analysis to determine possibly bioactive constituents. The antioxidant activity was carried out th
Química Nova. Publicado em: 2022
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5. MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting CST1
Abstract Objective: This study aims to explore the effect and mechanism of miR-375 in Laryngeal Squamous Cell Carcinoma (LSCC) cell progression. Methods: LSCC cells (LSC-1 and TU177) were transfected with miR-375-mimic, miR-375-inhibitor or miR-375-mimic + oe-CST1. The expression of miR-375, CST1, MMP-2, and MMP-9 was measured. The effect of miR-375-mimic,
Brazilian Journal of Otorhinolaryngology. Publicado em: 2022
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6. ASSESSMENT OF α-AMYLASE INHIBITION ACTIVITY BY AN OPTIMIZED AND VALIDATED IN VITRO MICROSCALE METHOD
Metabolic disorders, including hyperglycemia, characterize type-2 diabetes. One of the treatment methods used for postprandial hyperglycemia includes using potential therapeutic agents to inhibit α-amylase activity. This study utilized fractional design and the simplex method to optimize in vitro microscale assay inhibition conditions using Miller’s react
Química Nova. Publicado em: 2022
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7. Linseed Oil Nanoemulsion with Pluronic® F127 Loaded with Betulinic Acid: Preparation, Rheology, MTT Assay and in vitro Release Kinetics
The main objective of this work was to develop a nanoemulsion based on linseed oil and betulinic acid, stabilized with Pluronic F127 and polyglycerol polyricinoleate, for anticancer applications. The nanoemulsions were synthesized by ultrasound and evaluated for in vitro cytotoxicity, particle size, polydispersity index, zeta potential, morphology, encapsula
Journal of the Brazilian Chemical Society. Publicado em: 2022
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8. Prevalence of Epstein-Barr Viral DNA among children at a single hospital in Suzhou, China
Abstract Objective: This study aimed to describe the prevalence of Epstein-Barr virus (EBV)-DNA among children in Suzhou, and to explore the association between plasma EBV load and disease diagnosis. Methods: All children admitted to the Children’s Hospital of Soochow University between January 2018 and September 2020 and subjected to the plasma EBV-DNA
Jornal de Pediatria. Publicado em: 2022
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9. Benzophenone Derivatives Showed Dual Anti-Inflammatory and Antiproliferative Activities by Inhibiting COX Enzymes and Promote Cyclin E Downregulation
Considering the promising antitumor effects of compounds with dual anti-inflammatory and antiproliferative activities, thus benzophenones analogs (2-7) were evaluated on in vivo anti-inflammatory assay and molecular docking analysis. Those with the best molecular docking results were in vitro evaluated on cyclooxygenase (COX) enzymes and tested regarding ant
Journal of the Brazilian Chemical Society. Publicado em: 2022
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10. Synthesis and SARS-CoV-2 3CL Protease Inhibitory Effects of Oxazolidinone Derivatives
The 3-chymotrypsin-like protease (3CLpro) is an attractive target for the development of anti-SARS (severe acute respiratory syndrome) drugs. In this work, a series of oxazolidinone derivatives 3a-3v were synthesized and their inhibitory activities against SARS coronavirus 2 (SARS-CoV-2) 3CLpro were evaluated by the fluorescence resonance energy transfer (FR
Journal of the Brazilian Chemical Society. Publicado em: 2022
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11. Characterization of Commercially Available Turmeric for Use in Pharmaceutical Products and Food Supplements
We addressed some physicochemical and biological differences in commercial turmeric, acquired from both handling pharmacies and stores selling natural products for quality assessment. For this, it was determined the melting point, antioxidant activity by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), antimicrobial activity via the agar disk-diffusion assay,
Journal of the Brazilian Chemical Society. Publicado em: 2022
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12. Mortality of Diatraea saccharalis is affected by the pH values of the spore suspension of Beauveria bassiana and Metarhizium anisopliae
ABSTRACT Fungal virulence is multifaceted and dependent on multiple factors including the pH of the spore suspension. In this study, we accessed effects of six pH values of Beauveria bassiana, and Metarhizium anisopliae medium for the growth, sporulation, and mortality on sugarcane stalk borer Diatraea saccharalis. The culture of fungi was performed onplates
Revista Ceres. Publicado em: 2022