VARIABILIDAD GENÉTICA DE PARENTALES Y POBLACIONES F1 INTER E INTRAESPECÍFICAS DE Physalis peruviana L. Y P. floridana Rydb.

AUTOR(ES)
FONTE

Rev. Bras. Frutic.

DATA DE PUBLICAÇÃO

2015-03

RESUMO

ABSTRACTCape gooseberry, Physalis peruviana, is an Andean fruit of great importance for export markets; its main limitation for production in Colombia is the vascular wilt caused by Fusarium oxysporum. The present study proposed the generation of F1 populations between contrasting pathogen response parents and their evaluation at molecular level to support knowledge and use of the species genetics resources. To do this, four genotypes of P. peruviana, and one of the related species P. floridana, were characterized at morphological level using 34 qualitative and 20 quantitative variables and at molecular level using 328 COSII and 154 IRGs markers. The genotypes were used as parents for generation and molecular characterization of F1 populations. Quantitative variables were able to distinguish the species P. floridana and P. peruviana as well as cultivated and wild genotypes within P. peruviana. One hundred percent viability was found in intraspecific F1 crosses and 50% in interspecific crosses, remaining viable only when P. floridana was the receptor of pollen. Molecular characterization did not identify polymorphisms within the P. peruviana but between P. floridana and P. peruviana. An F1 population of 51 individuals generated between the species a total of 127 alleles with an average of 3.18 per locus, a PIC of 0.358 and high values of heterozygosity (Ho: 0.737 and He: 0.449). PCA and cluster analysis allowed discrimination of the F1 population in three groups, more similar to the P. floridana parental. This was reflected by a 75% Mendelian distortion favored by the presence of 63.75% maternal alleles. The study provides insights into Cape gooseberry crossability and the genetic variability of parental genotypes and F1 populations.

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