TRIAZENES: DNA CLEAVAGE, ANTIBACTERIAL ACTIVITY AND TOXICITY FRONT TO Artemia Salina / TRIAZENOS: CLIVAGEM DO DNA, ATIVIDADE ANTIBACTERIANA E TOXICIDADE FRENTE À Artemia Salina LEACH.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

In this work, six triazene compounds are assayed to DNA cleavage activity. The same six triazenes and the drug Asercit (dacarbazine) are assayed to antibacterial activity and toxicity to Artemia salina Leach.: 1,3-bis-(phenyl)triazene-1-N-hidroxide (T1), 1-(4-bromophenyl)-3-(4-nitrophenyl)triazene, (T2), 1-(4-azophenyl)-3-(4-nitrophenyl)triazene (T3) ,1,3-bis-(4-azophenyl-triazene) (T4), 1,3-bis-(2-bromophenyl)triazene (T5), 1-(4-carboxyphenyl)-3-(4-azophenyl)triazene (T6) and 5-(3,3-dimethyl-1-triazenyl)imidazol-4-carboxamide (dacarbazine, Asercit). Triazene T1 cleaves approximately 50 % of plasmid DNA (pBSKII and pUC18, 3.75 mM, 50C/24 hours, Tris.HCl buffer 200 mM pH 8.0). Hydroxyl radical scavengers (glycerol 0.1 and 1.0 %, DMSO 0.04 M and tiourea 0.04 M) and argon atmosphere not interfere in DNA cleavage by T1. The Kb of T1 determinated by spectrofotometric titrations with DNA are 4.50 x 101 M-1 (pH 6,5), 1.00 x 102 M-1 (pH 7,0) e 2.33 x 102 M-1 (pH 8,0). T1, T6 and Asercit are the more actives in antibacterial activity with CIM/CBM of even 16/64 μg/ml. LC50 more lower determinated by toxicity to A. salina are to T1 (0.081 0.008 μg/ml), T5 (0.076 0.011 μg/ml) and T6 (0.077 0.007 μg/ml). Theses studies show the wide biological activity conferred by triazene compounds.

ASSUNTO(S)

clivagem do dna dna cleavage antibacterial activity triazenos atividade antibacteriana artemia salina. farmacia artemia salina triazenes

ACESSO AO ARTIGO

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