Transformação genética de maracujá amarelo visando resistência à Xanthomonas axonopodis pv. passiflorae / Genetic transformation of yellow passion fruit to confer resistance to Xanthomonas axonopodis pv. passiflorae

AUTOR(ES)

Mariza Monteiro

DATA DE PUBLICAÇÃO

2005

RESUMO

Bacterial spot disease caused by Xanthomonas axonopodis pv. passiflorae is a serious problem in many passion fruit production areas in Brazil, especially if associated with anthracnose. Genetic transformation provides an alternative for obtaining resistant plants. Bactericide proteins such as attacins, found in the haemolymph of insects, have been used to confer resistance on plant species. As the attacins have a sign peptide that dispatches them to extracellular space in insects, we initiated our studies investigating the attacin A directing in plants. The attacin A gene (attA) sequence, with and without the sign peptide, was fused to uidA and gfp reporter genes, and onion epidermis were transformed using bioballistics with gene constructions. The protein did accumulate in the apoplast, where bacteria multiply before attacking plant cells. With the aim of obtaining transgenic plants of yellow passion fruit resistant to bacterial disease, leaf and hypocotyl-derived tissues were transformed with LBA 4404 and EHA 105 strains of Agrobacterium tumefaciens containing the attA gene. From a total of 313 infected explants, we obtained 31 PCR+ shoots, a transformation efficiency of 10%. Expression of the attA gene was confirmed by RT-PCR, and pathogen resistance evaluated by X. axonopodis pv. passiflorae inoculation in leaves obtained from in vitro plants. Leaf lesions were not observed in 10 shoots, suggesting a possible resistance to pathogen.

ASSUNTO(S)

proteína de planta xanthomonas transformação genética agrobacterium agrobacterium passion fruit plant protein genetic transformation bacteriose vegetal maracujá plant disease

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