Agrobacterium
Mostrando 1-12 de 1414 artigos, teses e dissertações.
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1. Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses
Abstract Drought and cold are the primary factors limiting plant growth worldwide. The Ammopiptanthus mongolicus NAC11 (AmNAC11) gene encodes a stress-responsive transcription factor. Expression of the AmNAC11 gene was induced by drought, cold and high salinity. The AmNAC11 protein was localized in the nucleus and plays an important role in tolerance to drou
Genet. Mol. Biol.. Publicado em: 14/11/2019
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2. Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
Abstract Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries the binary plasmid pBIN19::Rd29A::ScTPS1TPS2::nos and pBIN19::35S::ScTPS1TPS2::nos, respectively. ISSR markers were used to discriminate two cultivars from each other, and independent transgenic plants gener
Crop Breed. Appl. Biotechnol.. Publicado em: 31/10/2019
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3. Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resist
Genet. Mol. Biol.. Publicado em: 13/06/2019
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4. Molecular farming of antimicrobial peptides: available platforms and strategies for improving protein biosynthesis using modified virus vectors
Abstract The constant demand for new antibiotic drugs has driven efforts by the scientific community to prospect for peptides with a broad spectrum of action. In this context, antimicrobial peptides (AMPs) have acquired great scientific importance in recent years due to their ability to possess antimicrobial and immunomodulatory activity. In the last two dec
An. Acad. Bras. Ciênc.. Publicado em: 18/10/2018
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5. Aumento da transformação genética transiente em embriões imaturos do genótipo brasileiro de milho BR 451 co-cultivados com Agrobacterium tumefaciens
RESUMO. A engenharia genética ampliou as possibilidades do melhoramento genético vegetal, apoiando ideais de sustentabilidade. A Agrobacterium tumefaciens é o sistema de transformação preferido, uma vez que permite a produção de plantas transgênicas com maior estabilidade na expressão gênica e na herdabilidade. A Agrobactéria e o tecido vegetal de
Acta Sci., Agron.. Publicado em: 16/07/2018
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6. Rhizosphere Bacterial Composition of the Sugar Beet Using SDS-PAGE Methodology
ABSTRACT The rhizosphere zone has been defined as the volume of soil directly influenced by the presence of living plant roots or soil compartment influenced by the root. During the growing season of 2014, the rhizobacteria of 23 sugar beet plants sampled from 12 sites in the west and north west of Iran were inventoried. Using a cultivation-dependent approac
Braz. arch. biol. technol.. Publicado em: 14/06/2018
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7. pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 bin
Genet. Mol. Biol.. Publicado em: 11/06/2018
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8. OTIMIZAÇÃO DE FATORES QUE AFETAM A TRANSFORMAÇÃO GENÉTICA DE Eucalyptus saligna MEDIADA POR Agrobacterium tumefaciens
RESUMO Esse trabalho teve como objetivo avaliar o efeito de vários fatores que podem afetar a transformação genética de explantes cotiledonares de Eucalyptus saligna mediante co-cultura com a cepa EHA105 de Agrobacterium tumefaciens. Foi utilizado o vector pBI121 carregando o gene repórter gus sob controle do promotor 35SCaMV. Foi estudado o efeito dos
Rev. Árvore. Publicado em: 22/02/2018
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9. Beneficial effects of bio-controlling agent Bacillus cereus IB311 on the agricultural crop production and its biomass optimization through response surface methodology
ABSTRACT Disease in agricultural field is a big problem that causes a massive loss in production. In this present investigation, we have reported a soil-borne bacterium Bacillus cereus IB311 which is antagonistic to plant pathogens (Pseudomonas syringae and Agrobacterium tumefaciens), and could make a substantial contribution to the prevention of plant disea
An. Acad. Bras. Ciênc.. Publicado em: 16/10/2017
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10. Transformação genética da variedade brasileira de milho BR 451 pelo método da Agrobacterium tumefaciens
RESUMO: A introdução de genes de forma assexual por meio da engenharia genética tem ampliado as possibilidades do melhoramento genético vegetal. No entanto, devido principalmente a baixa resposta in vitro, a transformação genética tem se limitado a poucos genótipos das espécies de interesse agronômico. Visando estabelecer protocolo de transformaç
Cienc. Rural. Publicado em: 09/10/2017
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11. Enzimas degradantes de organofosforados: Base molecular e perspectivas para biorremediação enzimática de agroquímicos
RESUMO Muitos compostos organofosforados (OP) são utilizados até hoje na agricultura como pesticidas e, infelizmente, como agentes de guerra química (ou agentes dos nervos) também. Os pesticidas organofosforados e os agentes dos nervos são moléculas extremamente tóxicas, uma vez que atuam como inibidores da enzima Acetilcolinesterase (AChE). O efeito
Ciênc. agrotec.. Publicado em: 2017-09
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12. Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
Abstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-09