Transformação do fungo ocratoxigênico Aspergillus westerdijkiae medida por Agrobacterium tumefaciens

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Aspergillus westerdijkiae is a potent ochratoxin A producer fequently associated to coffee beans. Ochratoxin A is known to have nephrotoxic effects and carcinogenic potential in animal species. Here we report the first Agrobacterium-mediated transformation of A. westerdijkiae. Conidia were transformed to hygromicin B resistance using AGL-1 strain of Agrobacterium tumefaciens. The most favourable conditions allowed a mean transformation frequency of 36 transformants per 106 target conidia. Among 600 transformants 30 (5%) showed morphological alterations. Two transformants (T10 and TS18) with consistently reduced ochratoxin A production were obtained. Both of then also showed significant reduction in conidiogenesis compared with the wild type. Fungal sequences flanking the insertion site could be amplified by Thermal Asymmetric Interlaced PCR. The sequence obtained from tarnsformant TS18 had similarity to a monooxygenase putative from A. fumigatus. Transformant T10 had a sequence with similarity to a jumonji family of transcription factor from A. fumigatus.

ASSUNTO(S)

aspergilos agrobacterium microbiologia genética vegetal aspergillus agrobacterium microbiology plant genetics

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