Senescence of Pear Fruit Cells Cultured in a Continuously Renewed, Auxin-deprived Medium
AUTOR(ES)
Pech, Jean-Claude
RESUMO
Auxins and cytokinins support cell division in tissue and cell cultures. In cytokinin-independent pear (Pyrus communis) cells, omission of 2,4-dichlorophenoxyacetic acid (2,4-D) from the medium for two successive transfers leads to rapid cell lysis, unless the osmolarity is raised to 0.4 molar with mannitol. Use of this system (nutrients plus mannitol minus 2,4-D) for the study of cell senescence was explored both in batch culture and in a system designed to permit medium renewal without withdrawal of live cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=543369Documentos Relacionados
- Some Physiological Changes Occurring during the Senescence of Auxin-Deprived Pear Cells in Culture 1
- Protein Synthesis Associated with Quiescence and Senescence in Auxin-Starved Pear Cells 1
- Polyribosomes from Pear Fruit: Changes during Ripening and Senescence
- Effect of High Temperature on Calcium Uptake by Suspension-Cultured Pear Fruit Cells
- Polyribosomes from Pear Fruit: II. CHANGES OCCURRING IN PULP TISSUES DURING RIPENING AND SENESCENCE