Effect of High Temperature on Calcium Uptake by Suspension-Cultured Pear Fruit Cells

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RESUMO

Uptake of Ca2+ by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38°C compared to 25°C. The increased uptake was specific for Ca2+ and was not due to cell wall binding. Tissues pretreated at 38°C showed increased uptake even upon return to 25°C. Treatment with carbonylcyanide m-chlorophenylhydrazone, salicylhydroxamic acid + KCN, or arsenite also increased Ca2+ content of cells. Results are discussed with regard to membrane permeability changes, the cellular control of Ca2+, and heat treatments used to inhibit softening of fruit during postharvest storage.

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