Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.
AUTOR(ES)
McKeehan, W L
RESUMO
The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a source of selenium in most culture media, since higher concentrations of serum protein or whole serum mask the selenium requirement of WI-38 cells. Selenium is also required by a Chinese hamster cell line that can be grown in a protein-free synthetic culture medium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=430440Documentos Relacionados
- Activation of histone gene transcription by nonhistone chromosomal proteins in WI-38 human diploid fibroblasts.
- Dual pathways for ribonucleic acid turnover in WI-38 but not in I-cell human diploid fibroblasts.
- Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38
- Expression of cell cycle-dependent genes in young and senescent WI-38 fibroblasts.
- Transforming growth factor-beta is a strong and fast acting positive regulator of the level of type-1 plasminogen activator inhibitor mRNA in WI-38 human lung fibroblasts.