Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38

AUTOR(ES)

Reitman, Morton

RESUMO

We demonstrated that Venezuelan equine encephalomyelitis (VEE) virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 109.8 mouse intracerebral 50% lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects.

Documentos Relacionados

• Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.
• Dual pathways for ribonucleic acid turnover in WI-38 but not in I-cell human diploid fibroblasts.
• Activation of histone gene transcription by nonhistone chromosomal proteins in WI-38 human diploid fibroblasts.
• Propagation of Rhinovirus on WI-38 Cell Monolayers in Rolling Bottles
• Morphogenesis of Venezuelan Equine Encephalomyelitis Virus