Activation of histone gene transcription by nonhistone chromosomal proteins in WI-38 human diploid fibroblasts.
AUTOR(ES)
Jansing, R L
RESUMO
The regulation of histone gene expression was examined after confluent, nondividing WI-38 human diploid fibroblasts were stimulated to proliferate. Histone mRNA sequences were assayed by hybrid formation with an 3H-labeled single-stranded DNA complementary to histone mRNAs. Histone mRNA became associated with polyribosomes concomitant with the activation of DNA synthesis. The ability of chromatin from WI-38 cells to serve as a template for in vitro transcription of histone mRNA sequences parallels the onset of DNA replication. A role for nonhistone chromosomal proteins in the control of histone gene readout is suggested because, when chromatin from confluent WI-38 cells was dissociated and then reconstituted in the presence of S phase nonhistone chromosomal proteins, a 500-fold activation of histone mRNA sequence transcription was observed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393220Documentos Relacionados
- Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.
- Dual pathways for ribonucleic acid turnover in WI-38 but not in I-cell human diploid fibroblasts.
- Expression of cell cycle-dependent genes in young and senescent WI-38 fibroblasts.
- Gene Activation in WI-38 Fibroblasts Stimulated to Proliferate: Requirement for Protein Synthesis
- Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38
