Quasispecies detection in human respiratory syncytial virus (HRSV) samples in absence and presence of polyclonal serum / Detecção de quasispecies em amostras de vírus respiratório sincicial humano (HSRV) na ausência e na presença de soros policlonais

AUTOR(ES)

Claudia Trigo Pedroso de Moraes Sales

DATA DE PUBLICAÇÃO

2009

RESUMO

Human respiratory syncytial virus (HRSV) is one of the most important clinical respiratory pathogens, since 64 millions children in the world are infected by this agent every year. Host immunity and viral genetic variability are important factors to a vaccine development, besides quasispecies presence in the viral population. In this work, HRSV quasispecies were detected in clinical samples in absence and presence of human polyclonal serum collected by children in the convalescent phase and mother serum. A non-synonymy variation was found in the F gene in antibodies absence. Four mutations were found at HRSV G2 in polyclonal serum absence. Two were synonymy and two were non-synonymy variation, the last in the same nucleotide. A non-synonymy mutation was found in the G2 region in presence of polyclonal serum collected from child convalescent phase. This alteration was the same of the observed in absence of polyclonal serum so it is possible that host antibodies can selected different viral minority sequences present in the population.

ASSUNTO(S)

human polyclonal serum proteína f vírus respiratório sincicial human g protein soro policlonal humano plaqueamento quasispecies human respiratory syncytial virus plaque assay proteína g f protein quasispecies

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