PREGNANCY RATES USING FROZEN SEMEN OF CRIOLLO STALLIONS WITH GLYCEROL OR DIMETHYLFORMAMID AS CRYOPROTECTANTS / ÍNDICE DE PRENHEZ COM SÊMEN CONGELADO DE GARANHÕES CRIOULOS USANDO GLICEROL OU DIMETILFORMAMIDA COMO CRIOPROTETORES
AUTOR(ES)
Rodrigo Arruda de Oliveira
DATA DE PUBLICAÇÃO
2007
RESUMO
During the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of 30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallions semen. In this context DMF brought better results.
ASSUNTO(S)
medicina veterinaria glicerol dimetilformamida criollo eqüino dimethylformamid glycerol frozen semen sêmen congelado crioulo equine
ACESSO AO ARTIGO
http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3042Documentos Relacionados
- Índice de prenhez com sêmen congelado de garanhões da raça Crioula usando glicerol ou dimetilformamida como crioprotetores
- Uso de glicerol, metil-formamida e dimetil-formamida como crioprotetores do sêmen canino
- Uso da dimetil-formamida associada ou não ao glicerol na criopreservação de sêmen caprino
- Uso de dimetil-formamida associada ou não ao glicerol na criopreservação de sêmen caprino
- Uso de diferentes crioprotetores em diluentes para sêmen ovino congelado