Potencial Antitumoral de extratos da própolis brasileira e de folhas de graviola (Annona muricata): efeito citotóxico sobre células hepatocarcinogênicas HepG2 / Anti-tumorous potencial of Brazilian propolis and soursop leaf extract (Annona muricata): cytotoxic effect on hepatic carcinogenic HepG2 cells

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

19/02/2010

RESUMO

The hepatocellular carcinoma is the most common adult primary liver câncer and it has already become the fifth most common neoplasia in the world and the third in number of deaths related to cancer. It is estimated that 2/3 of all human neoplasias could be prevented by a change in lifestyle, including a diet. The propolis, elaborated from various plant parts by Apis mellifera bees, present pharmacological properties effective in cancer treatment by biological activity attributed to its chemical components, mainly with the Brazilian propolis. More than 300 propolis compounds have been identified. Recently pharmacological studies, in which scientific interest is focused on the acetogenins, a compound class derived from fatty acids and presenting a strong anti-tumorous activity. Three experiments were carried out in the Animal Molecular Infectology Lab, in the Biochemistry and Molecular Biology Department, in the Federal University of Viçosa, for testing in vitro, the anti-tumorous properties of soursop extracts (Annona muricata) and green and red propolis extracts. For so, tumorous hepatic cells from the HepG2 lineage were used, cultivated in four different concentrations of soursop extracts (1, 2, 4 e 8 mg/mL) and red propolis (25, 50, 100 and 200 μg/mL) and green extracts (50, 100, 200 and 400 μg/mL), with espectrophotometry analysis of the cellular viability by the methodology of neutral red dye, in three time periods (24, 48 and 72 hours). The cells wich were exposed th the treatments showed strong inhibition to proliferation, mainly in the concentration of 8 mg/mL of soursop extract (86% after 24 hours and 95% after 48 hours), 200 μg/mL of red propolis extract (92% after 24 hours and 100% after 48 hours) and 400 μg/mL of green propolis extract (92% after 72 hours), which caused visible alterations in the cellular architecture as well as in the single-layer cultivation organization, showing abundant apoptotic forms. The chromatographic analysis of the chemical profile of green and red propolis extracts revealed the presence of flavonoids and other compounds with known anti-tumorous effect, such as artepellin C, pinocembrin, pinobankisin, quercetina, kanpherol, chrysin, galangin and bacarin. The main conclusion to be drawn from this discussion is that the soursop (A. muricata) along with the green and red propolis are sources of cytotoxic compounds with pontentical against hepatic carcinogenics HepG2 cells.

ASSUNTO(S)

extratos da própolis graviola células hepatocarcinogênicas hepg2 metabolismo e bioenergetica propolis extract soursop hepatic carcinogenic hepg2 cells

ACESSO AO ARTIGO

Documentos Relacionados