Pneumovirus aviario (PVA): detecção por RT-PCR quimioluninescente e caracterização dos isolados brasileiros por analise com enzimas de restrição e sequenciamento

AUTOR(ES)

Maria Angela Gomes de Castro Dani

DATA DE PUBLICAÇÃO

1998

RESUMO

Avian Pneumovirus (APV) causes an acute respiratory tract infection both in turkeys (turkey rhinotracheitis) and chicken (swollen head syndrome) with sudden onset and rapid spread through the flocks. In this study, immunochemiluminescent Southern blot RT-PCR assay was employed to detect a F gene transcript of the avian pneumovirus (APV) in two European turkey isolates and two Brazilian chicken isolates. Limiting dilution PCR was performed to compare the sensitivity of immunochemiluminescent Southern blot assay and nested PCR (nPCR) assay. The sensitivity and specificity of immunochemiluminescent Southem blot RT-PCR assay were comparable to that of nPCR, and at least 100 fold more sensitive than a single PCR amplification. Sequence analysis of the 175 bp product ofthe F gene revealed 100% identity with earlier reported PVA sequences. We characterized the Brazilian isolates using restriction analysis and sequencing of a 600 bp fragment of the G gene and conclude that the isolates belong to the APV subgroup A

ASSUNTO(S)

virologia reação em cadeia de polimerase biologia molecular

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