Caracterização de isolados brasileiros de Pneumovirus Aviario (PVA)

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

Avian Pneumovirus (APV) causes an acute respiratory tract infection both in turkeys (turkey rhinotracheitis) and chicken (swollen head syndrome) with sudden onset and rapid spread through the flocks. In this work were characterized two types of avian pneumovirus from brazilian chicken by electron microscopy, serological analysis (serum neutralization and ELISA tests), physico-chemical properties (thermostability, chloroform sensitivity, effect of IUDR and pH, haemagglutination and haemodsortion tests) and compared its polypeptide composition with four other avian pneumoviruses using SDSP AGE. AlI isolates from APV were propagated in cells cultures of CER for antigens production with titre varying from 105.5 to 106.5 TCID50/ml. Only one isolated showed ciliostasis after the second passage. The physico-chemical tests and morphological observation showed that brasilian s isolates are pleomorphic RNA viruses, spicular, enveloped, thermolabile and not haemagglutinating. The SDS-PAGE profile of the viruses were very similar. Virus neutralisation tests revealed non reaction of the two brazilian isolates with the other different isolates. Nine hundred one serum samples of breeders and seven hundred twenty four serum samples of broilers were analysed using the serological tests of serum neutralization (SN) and enzyme-linked immunosorbent assay (ELISA) and both tests showed serum positivity, respectively, 66.5 and 67% to breeders and 5.1 and 2.9 to broilers. In the experimental inoculation of SPF birds with two brazilian isolates and one germany isolated observed serological conversion after the first inoculation

ASSUNTO(S)

eletroforese ave virus

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