Photometric procedure for quantitative analysis of Aflatoxin B1 in peanuts by thin-layer chromatography using charge coupled device detector

AUTOR(ES)

Hoeltz, Michele, Welke, Juliane Elisa, Noll, Isa Beatriz, Dottori, Horacio Alberto

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2010

RESUMO

A photometric procedure was developed for determination of aflatoxin B1 in peanuts by TLC-CCD technique. The quantification and detection limit were 1.2 μg kg-1 and 0.4 ng per spot, respectively, with mean recovery of 98%. The CCD camera is sufficiently sensitive to detect small changes in spots fluorescence intensity and the results for performance confirmed the efficiency of the method. Another important property of CCD detector is its linearity for a wide range of luminous stimulus determined by analysis of five-point calibration curves using the intensity of AFB1 fluorescence versus AFB1 concentration (0.8 to 4.8 ng per spot). The method was applied to the analysis of thirty nine peanut samples and aflatoxin B1 levels ranged from 16 to 115 μg kg-1. The TLC-CCD and the photometric procedure developed in this study demonstrated to be a simple and efficient tool for quantitative analyses of AFB1 in peanut samples.

Documentos Relacionados

• Determination of ochratoxin A in wine by high-performance thin-layer chromatography using charged coupled device
• Determination of ochratoxin a in wine by high-performance thin-layer chromatography using charged coupled device
• Clinical Analysis by Thin-Layer Chromatography Techniques
• Identification of barbiturates by thin-layer chromatography
• A simple apparatus for thin-layer chromatography