Paracoccidioidomicose experimental : infecção e doença em animais infectados por um isolado atipico da cepa Pb18 / Experimental Paracoccidioidomycoses infection and disease in animals infected by an atypical isolated of Pb18 strain

AUTOR(ES)

Paula Cristina de Souza Souto

DATA DE PUBLICAÇÃO

2006

RESUMO

P. brasiliensis is a dimorphic fungus that grows in mycelial phase at room temperature and in yeast form at 35-37°C. Recently, in the Immunopathology Laboratory of the Department of Microbiology and Immunology (Biology Institute/Unicamp), it was observed that a sample of the virulent strain Pb18 grew in a different way from other ones. Preliminary analysis of this culture showed that the isolated exhibited, exclusively, an intermediate form of pseudohyphae. That way, the objective of this work was the partial characterization of the atypical isolated (Pb18PH) through the comparison with the wild strain Pb18. For this, there were analyzed: the morphology of the isolated to 25 and 37ºC; the composition of cell wall proteins; the disease development and the adaptative immune response in BALB/c mice. The results showed that Pb18PH didn t alter their growth form, independently of the cultivation temperature or the passage through animal. By the analysis of the cell wall proteins, it was observed that Pb18PH didn t express some proteins and presented low concentration of the glicoprotein 43 (gp43) when compared with the wild one. The infection caused by this atypical isolated was softer than that observed with the virulent strain Pb18. Besides, it was noted the complete resolution of disseminated lesions. However, it was not possible to determine differences in the adaptative immune response evaluation of the animais infected with the different isolated. To despite the need of more studies about this atypical isolated, our results indicate that Pb18PH would come to be an important tool for the study of the fungus biology and their relationship with the host

ASSUNTO(S)

paracoccidioides brasiliensis mutation paracoccidioidomicose paracoccidioides brasiliensis biology paracoccidioidomycosis mycology micologia mutação (biologia) immune response resposta imune

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