Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Paracoccidioidomycosis (PCM) presents three major forms: PCM-infection (PI); adult form (AF) and juvenile form (JF). The aim of this study was to compare the immunological response in these groups through the analysis of the kinectics of mRNA expression of some cytokines and chemokines (IL-2, IL-4, IL-5, IL-6, IL-lO, IFN-y, TGF p, TNF-a, CXCLS, CXCL9 and CXCLlO) by RT-PCR, as well as, to characterize the phenotype of the cytokines producing cells by flow cytometry. The results showed that PI individuaIs express earlier and higher levels of mRNA for IFN-y, TNF-a, CXCL9 and CXCLIO when compared to JF patients and similar levels of CXCLlO and IFN-y and higher levels of CXCL9 and TNF -a when compared to AF ones. On the other hand, the mRNA expression ofTh2 cytokines (IL-4, IL-IO, IL-5 and TGF-P) was higher and earlier in JF and AF groups when compared to PI individuaIs. In relation to protein expression, we observed that PI group presents a higher percentage of cells producing IFN-y, TNF-a, IL-2, CXCL9 and CXCLIO when compared to both groups ofpatients. The production ofIFN-y was predominantIy done by CD3+CDS+ T cells, whereas IL-2 and TNF-a production was higher in CD3+CD4+ cells. Monocytes ofPI individuaIs also presented higher expression of CDSO and lower expression of CDS6 and CD54 when compared to JF and AF patients, and higher expression of HLA-DR and CDIIa only when compared to JF patients. These results indicate that the differential kinetic of mRNA expression of ThI or Th2 cytokines observed in the different forms of PCM may modulate the immunological response resulting in the different outcomes observed in this disease

ASSUNTO(S)

imunologia celular citocinas quimiocinas paracoccidioidomicose

ACESSO AO ARTIGO

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