OCHRATOXIN A AND CITRININ IN RICE: STUDY OF METHODOLOGY FOR SIMULTANEOUS DETERMINATION OF OCCURRENCE / OCRATOXINA A E CITRININA EM ARROZ: UM ESTUDO DE METODOLOGIA PARA DETERMINAÇÃO SIMULTÂNEA DE OCORRÊNCIA

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Ochratoxin A and citrinin are mycotoxins found naturally in foods and feeds. The evaluation of the risk of the consumption of contemned foods for those compositions should be dear starting from reliable data that reflect the true concentration of these toxins in different foods or inputs, especially if ingested frequently. This generates the need of necessary analytical methods that they are fast without disrespecting the classic stages of evaluation of lines, representative sampling, extraction, cleaning, concentration, separation in chemical ways, detection, identity confirmation and quantification. For the mycotoxins the techniques chromatography are in general the most applied and told in view of the structural diversity of these composed. In this work an extraction optimization was accomplished using the method QuEChERS modified in comparison with the methods Soares and Rodrigues Amaya (1989), Tanaka (2001) and Ultrasound (Palma et. al., 2007) using different techniques chromatography with different detectors for analysis of these mycotoxins simultaneously. The 38 samples of rice were analyzed cultivated and stored in experimental fields of Cachoeirinha in the South of Brazil. The use of the systems TLC, HPLC-PDA and LC-MS, provided specificity, precision and sensibility, so that the detection limits and quantification, they obtained inferior values to the established maximum limit for organs international regulators (5 ppb for ochratoxin A). The detection limits found for citrinin and ochratoxin A in thin layer were 4,7 and 6 times larger than for liquid chromatography of high efficiency coupled to detector of arrangement of diodes, 14 and 300 times larger than liquid chromatography coupled to detector of mass. The limits of quantification of the two micotoxinas were inside of the demanded by the European Legislation for OTA of 5 μg Kg-1 for HPLC-DAD and LCMS. In the thin layer chromatography that value was 4 times above the established for ocratoxina A. The occurrence of ocratoxina A and citrinin was verified in 16% of the samples being the tenors varying between 3 and 560 μg Kg-1, suggesting possible chronic exhibition the these mycotoxins in case the samples are consumed.

ASSUNTO(S)

ocratoxina a arroz ochratoxin a citrinin citrinina rice quimica analitica

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