Moniliophthora perniciosa genome: assembly and annotation of mitochondrion and development of a semi-automatic system of genes annotation / Genoma de Moniliophthora perniciosa : montagem e anotação da mitocondria e desenvolvimento de sistema de anotação semi-automatico de genes

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The mitochondrial genome (mtDNA) of the fungus Moniliophthora perniciosa was completely sequenced and it contains 109103 bases pair, with 31% of bases GC, smaller percentage than found in the sequences of the nuclear genome (47%). It is the largest mitochondrial genome of fungus described to the moment, and its size is consequence of great intergenic space, with several ORFs who can be confirmed as new genes. Computational analyses show the presence of variation in the number of mtDNAs / cell in different libraries, with significant tendency of smaller mtDNAs / cell number in group of libraries originating from cultures undergoes to repeatedly reply. Most of the typical genes (atp6, atp9, nad1-6, nad4L, cox1-3, cob, being the exception the atp8), all of the rRNAS, tRNAS (it was found at least one for each amino acid) and genes of the intronic ORFs are guided in the hourly sense. Surprisingly the mtDNA presents one region occupied for a structure of invertron, characteristic of plasmids kalilo-like, integrated in stable way to the genome in all of the varieties of the biotype C, and present in other tested biotypes. This sequence is available in the GenBank through the accession number: AY376688. The other work line was developed together with other bioinformatics of the Genomic and Expression Laboratory. Data mining and annotation of genes tools were developed for projects genome, being the largest prominences the Gene Projects, that allows mining and annotation of genes during the sequencing process, and the new annotation interface, developed to optimize the quality and the efficiency of the annotation of genes

ASSUNTO(S)

genome witches broom disease vassoura-de-bruxa (fitopatologia) bioinformatica mithocondria mitocondria bioinformatics genomas

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