Isolamento e identificaÃÃo de leveduras presentes em frutos tropicais e perfil eletroforÃtico de superÃxido dismutase

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Yeasts are unicellular microrganisms known mainly by the ability to ferment the substrates to obtain energy. They are the commonest contaminants of fruits in nature, causing significant losts in the fruit industries. However, these microrganisms are biotools largely used in science and in industries. As aerobic organisms, the yeasts use the superoxide dismutase (SOD) enzime as a defense mechanism against oxidative effects of free radicals. This antioxidant activity is the reason of high interest by SOD producing microrganisms. The objectives of this work were to isolate and taxonomic identificate the yeasts found in the tropical fruits acerola (Malpighia glabra) and gogoia (Solanum sp.) and compare its SOD expression. From samples of acerola from Olinda and JaboatÃo dos Guararapes and samples of gogoia from Parque Dois IrmÃos, localities of the Metropolitan Region of Recife City (PE State, Brazil), were obtained 472 yeasts isolates. A total of 49 isolates were taxonomically identified as belonging to eight genera: Brettanomyces, Kloeckera, Coccidiascus, Nadsonia, Dekkera, Saccharomycodes, Saccharomycopsis and Schizoblastosporion. The CuZnSOD profile for these isolates were established by native starch gel electrophoresis, revealing significative differences between the genera. The CuZnSOD profile can be used as diagnostic for the genera Saccharomycodes, Coccidiascus, Schizoblastosporion and Saccharomycopsis. This study begins the SOD characterization for these genera, contributing to the physiologic exploitation of these microrganisms

ASSUNTO(S)

frutos tropicais isolamento superÃxido dismutase genetica identificaÃÃo de leveduras

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