Interação entre proteínas fluorescentes e nanocristais de CdSe/ZnS / Interaction between fluorescent proteins and CdSe/ZnS nanocrystals

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Proteins belonging to the GFP family were used to characterize their interaction with fluorescent CdSe/ZnS nanocrystals and to verify the occurrence of resonance energy transfer (FRET) among these elements. Two donor-acceptor pairs were established, one having a protein as donor and the other having a nanocrystal as donor. In both cases the donor suffers quenching of its fluorescence after the formation of a complex with the acceptor. The complex formation is dependent on pH and is due to electrostatic interaction. It was possible to prove the occurrence of FRET between CdSe/ZnS nanocrystals emitting green fluorescence as donors and the protein HcRed1 as acceptor, through the detection of sensitized emission and anisotropy reduction. Apparent donor-acceptor distances were determined from efficiency measurements and Förster distances. The obtained distances agreed with the protein and nanocrystal dimensions

ASSUNTO(S)

nanocrystals nanocristais fret gfp hcred1 fluorescent proteins hcred1 cdse/zns gfp proteÌnas fluorescentes cdse/zns fret

ACESSO AO ARTIGO

Documentos Relacionados