Fluorescent Proteins
Mostrando 1-12 de 1195 artigos, teses e dissertações.
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1. Candida albicans Clinical Isolates from a Southwest Brazilian Tertiary Hospital Exhibit MFS-mediated Azole Resistance Profile
Abstract: Candida albicans is the most frequent fungal species that causes infections in humans. Fluconazole is the main antifungal used to treat Candida infections, and its prolonged and indiscriminate use for the last decades are the most established causes which originated resistant strains. Fungal drug resistance is associated to alterations in ERG11 gen
An. Acad. Bras. Ciênc.. Publicado em: 29/07/2019
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2. BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation
ABSTRACT Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines. Methods: BthTX-I cytotoxicity was determined
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/07/2019
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3. Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resist
Genet. Mol. Biol.. Publicado em: 13/06/2019
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4. Antitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrest
Resumo As propriedades antitumorais de extratos de glândulas salivares de carrapatos ou proteínas recombinantes foram relatadas recentemente, mas pouco se sabe sobre as propriedades antitumorais dos componentes secretados da saliva. O objetivo deste estudo foi investigar o efeito in vitro da saliva bruta do carrapato duro Amblyomma sculptum sobre as linha
Rev. Bras. Parasitol. Vet.. Publicado em: 14/02/2019
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5. Intended and Unintended Consequences and Applications of Unnatural Interfaces: Oligo p-Phenylene Ethynylene Electrolytes, Biological Cells and Biomacromolecules
This short review focuses on an extended study of synthetic oligomericphenyleneethynylene (OPE) electrolytes that have been investigated for both their antimicrobial activity and their fluorescence sensing properties. In both cases, interfaces between these synthetic electrolytes and naturally occurring materials such as proteins, lipids, nucleic acids and c
J. Braz. Chem. Soc.. Publicado em: 2016-02
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6. Production of phycobiliproteins by Arthrospira platensis under different lightconditions for application in food products
There has been an increase in investment in research on new sources of natural pigments for food application. Some cyanobacteria can change the structures responsible for light harvesting and cellular processes according to the wavelength and light intensity. This phenomenon has been described as complementary chromatic adaptation. The present study aimed
Food Sci. Technol. Publicado em: 2015-04
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7. Role of Light and Dark Cycle and Different Temperatures in the Regulation of Growth and Protein Expression in Oscillatoria agardhii Strain
The cyanobacterium Oscillatoria agardhii was isolated from the fresh water Mawatha lake, Jaipur and was grown in Zarrouk's medium at 25 ± 2°C, illuminated with white fluorescent light at the intensity of 2 500 lux with 12:12 h light and dark photoperiod. The effects of photoperiod and temperature on the growth and protein expression by Oscillatoria agardhi
Braz. arch. biol. technol.. Publicado em: 2014-12
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8. Construction of three new Gateway® expression plasmids for Trypanosoma cruzi
We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an e
Mem. Inst. Oswaldo Cruz. Publicado em: 21/11/2014
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9. Bufotenine is able to block rabies virus infection in BHK-21 cells
Background Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills more than 55.000 people every year. It is caused by an enveloped single stranded RNA virus that affects the central nervous system, through an infection initiated by the muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, s
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 18/11/2014
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10. Trypan blue exclusion assay by flow cytometry
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and
Braz J Med Biol Res. Publicado em: 18/03/2014
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11. Expressão das proteínas citoesqueléticas actina e tubulina em células osteogênicas cultivadas sobre vidro e vitrocerâmica bioativos / Expression of the cytoskeletal proteins actin and tubulin in osteogenic cells cultured on bioactive glass-based surfaces
Bioactive glasses and glass-ceramics have been successfully applied in various therapeutic strategies to promote the formation of mineralized matrix in bone defects. The exposure of these materials to biological fluids results in chemical and topographical modifications that may affect the interactions of cells with the biomaterial surface, with potential ef
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/08/2012
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12. Retention of a recombinant GFP protein expressed by the yellow fever 17D virus in the E/NS1 intergenic region in the endoplasmic reticulum
The flaviviral envelope proteins, E protein and precursor membrane protein, are mainly associated with the endoplasmic reticulum (ER) through two transmembrane (TM) domains that are exposed to the luminal face of this compartment. Their retention is associated with the viral assembly process. ER-retrieval motifs were mapped at the carboxy terminus of these e
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-03