Gfp
Mostrando 1-12 de 1397 artigos, teses e dissertações.
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1. [Lentivírus falham na entrega de transgenes em zigotos bovinos após coincubação com espermatozoide, durante fertilização in vitro]
RESUMO As vantagens dos animais transgênicos têm sido demonstradas em diferentes aplicações, entretanto muitas metodologias usadas para gerar animais geneticamente modificados (GM) apresentam baixas taxas de eficiência. O objetivo deste estudo foi avaliar a entrega dos vetores lentivirais (VLs) em zigotos durante a fertilização in vitro (FIV), para ge
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2021-02
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2. [PROVISIONAL] Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
Abstract Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encoded a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, i
Genet. Mol. Biol.. Publicado em: 02/09/2019
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3. Effect of Glass Fiber Post Adaptation on Push-Out Bond Strength to Root Dentin
Resumo O objetivo neste estudo foi avaliar o impacto de diferentes diâmetros de pinos de fibra de vidro (PFVs) na resistência de união à dentina. Quarenta dentes humanos unirradiculares foram tratados endodonticamente e utilizados para cimentação de PFVs (White Post DC, FGM) com diferentes diâmetros (n=10): P1 - ø 1,6 mm coronal x 0,85 mm apical; P2
Braz. Dent. J.. Publicado em: 22/07/2019
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4. Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resist
Genet. Mol. Biol.. Publicado em: 13/06/2019
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5. Protein-protein interactions of the nicotinamide/nicotinate mononucleotide adenylyltransferase of Leishmania braziliensis
BACKGROUND Nicotinamide adenine dinucleotide (NAD) plays a central role in energy metabolism and integrates cellular metabolism with signalling and gene expression. NAD biosynthesis depends on the enzyme nicotinamide/nicotinate mononucleotide adenylyltransferase (NMNAT; EC: 2.7.7.1/18), in which converge the de novo and salvage pathways. OBJECTIVE The pu
Mem. Inst. Oswaldo Cruz. Publicado em: 21/03/2019
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6. Light curing resin cements containing iodonium salts promote suitable apical bonding of posts to radicular dentin
Abstract The aim of this study was to analyze the efficiency of experimental light-curing resin cements (ERCs) with a ternary photo-initiator system containing diphenyliodonium hexafluorphosphate (DPI) and different amines on retention of glass-fiber posts to dentin (GFP). ERCs formulations: a 1:1 mass ratio of 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phen
Braz. oral res.. Publicado em: 14/11/2018
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7. The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was indu
Int. braz j urol.. Publicado em: 2018-06
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8. Benefícios da condroitinase abc associada a células-tronco mesenquimais na lesão espinhal aguda em ratos
RESUMO Com o objetivo de estudar o efeito da condroitinase associada às células-tronco mesenquimais na lesão aguda da medula espinhal, utilizaram-se 50 ratos Lewis, distribuídos igualmente nos grupos: controle negativo (CN), tratamento com placebo (PLA), condroitinase (CDN), células-tronco mesenquimais (CTM) e condroitinase mais células-tronco mesenqui
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2018-06
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9. 4-Nerolidylcatechol induces autophagy in human glioblastoma cells
ABSTRACT Gliomas account for the majority of primary malignant brain tumors and present invasive behavior into adjacent healthy tissue. While 4-NC had previously shown to induce apoptotic cell death in a melanoma model, for the glioma model described in this paper 4-NC is cytotoxic for the cells with the induction of the autophagic pathway. Trypan blue exclu
Braz. J. Pharm. Sci.. Publicado em: 01/02/2018
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10. Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells
This study aimed to investigate the mechanism of hypoxia-inducible factor-1 alpha (HIF-1α) mediated hypoxia-induced permeability changes in bladder endothelial cells. Models of in vitro hypoxic cell culture of bladder cancer, bladder cancer cells with low HIF-1α expression and HIF-1α RNA interference (RNAi) expression vector were established. Western blot
Braz J Med Biol Res. Publicado em: 18/12/2017
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11. Células-tronco mesenquimais aplicadas nas fases inflamatória e proliferativa da cicatrização de feridas cutâneas
RESUMO A cicatrização de feridas é um processo que requer a interação de várias células da derme e epiderme. O objetivo deste trabalho foi avaliar qual o momento da aplicação das células das ADSCs em feridas cutâneas agudas que faria diferença na cicatrização nos primeiros sete dias da lesão. As células-tronco foram isoladas do tecido adiposo
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2017-11
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12. Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins
Abstract Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a
Genet. Mol. Biol.. Publicado em: 28/08/2017