Influence of the Lipid Phase Removal on the Quantification of Cu, Fe and Zn Bound to Proteins from Golden Flaxseed (Linum usitatissimum L .)
AUTOR(ES)
Marthe, Deyse de Brito, Augusti, Rodinei, Costa, Letícia Malta
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2015-08
RESUMO
The analysis of metals bound to proteins requires careful sample preparation. Experimental procedures involved in this area should be developed aiming to retain the metal species bound to the protein structure. Some pre-fractionation steps are commonly used and in many cases it is necessary to disrupt the lipid-protein interaction by performing lipid removal before protein extraction. This paper presents a systematic investigation on the influence of using hexane, chloroform and methanol neat or in sequence to remove the lipid fraction in golden flaxseed (
Documentos Relacionados
- Atividade antioxidante de produtos proteicos de linhaça (Linum usitatissimum L.)
- Heating effects on physicochemical characteristics and antioxidant activity of flaxseed hull oil (Linum usitatissimum L)
- Effect of seed maturation stages on physical properties and antioxidant activity in flaxseed ( Linum usitatissimum L.)
- GOMA DA LINHAÇA (Linum usitatissimum L.) PARA USO COMO HIDROCOLÓIDE NA INDÚSTRIA ALIMENTÍCIA
- PROPRIEDADES FUNCIONAIS DA LINHAÇA (Linum usitatissimum L.) EM DIFERENTES CONDIÇÕES DE PREPARO E DE USO EM ALIMENTOS