2015-08
Influence of the Lipid Phase Removal on the Quantification of Cu, Fe and Zn Bound to Proteins from Golden Flaxseed (Linum usitatissimum L .)
The analysis of metals bound to proteins requires careful sample preparation. Experimental procedures involved in this area should be developed aiming to retain the metal species bound to the protein structure. Some pre-fractionation steps are commonly used and in many cases it is necessary to disrupt the lipid-protein interaction by performing lipid removal before protein extraction. This paper presents a systematic investigation on the influence of using hexane, chloroform and methanol neat or in sequence to remove the lipid fraction in golden flaxseed (
