Identification of molecular marker linked to the Pm-1 gene that confers resistance to race 1 of to powdery mildew (Podosphaera xanthii) in melon (Cucumis melo L.) / Identificação de marcador molecular ligado ao gene Pm-1 que confere resistência a raça 1 de oídio (Podosphaera xanthii) em melão (Cucumis melo L.)

AUTOR(ES)

Fatima Aparecida da Silva-Barreto

DATA DE PUBLICAÇÃO

2007

RESUMO

Melon crop is of great economical importance in Brazil being the Northeast region the main producer and exporter of the fruit. The low levels of resistance to pathogens is one of the restricting factors for the Brazilian competitiveness worldwide. One of the most important diseases is powdery mildew caused by Podosphaera xanthii. Generally, the control of P. xanthii is achieved with the use of fungicides. However it is necessary to use less expensive control methods with a minimum environmental impact such as resistant cultivars. The objective of this work was to identify molecular markers linked to the Pm-1 gene which confers resistance to race 1 of P. xanthii with the purpose of assisting boding program. Two near isogenic lines (LQIs) of melon Agro AF426pm1 (P1) and AF426Pm1 (P2), both belonging to the inodorus variety, which are respectivally contrasting for abscence and presence of Pm-1 gene were analyzed. For the cosegregation analyses between gene and candidate markers, it was used the BC1F1 population was used screened for resistance to powdery mildew and obtained from a cross between the LQIs. The LM-PCR and AFLP techniques were efficient in the polymorphisms detection, however only those ones which were found using AFLP technique could be used as markers in the co-segregation test. It was found two markers with this technique but just the HF155 is located 4.9 cM of the Pm-1 gene. Due the proximity of the HF155 marker to the Pm-1 gene this one can be used in markerassisted selection aiming to develop melon cultivars resistant to P. xanthii.

ASSUNTO(S)

genes oídio molecular marker resistance genetic plant resistência genética vegetal powdery mildew gene melon marcador molecular melão

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