Genetic mapping of AFLP markers linked to Hemileia vastatrix Berk. &Br. resistance gene / Mapeamento genético de marcadores AFLP ligados ao gene de resistência do cafeeiro à Hemileia vastatrix Berk. &Br.

AUTOR(ES)

Giovani Greigh de Brito

DATA DE PUBLICAÇÃO

2007

RESUMO

Coffee leaf rust caused by the fungus Hemileia vastatrix is the most important disease of Coffea arabica. The objective of this study was to characterize the inheritance of coffee resistance gene to race II of this pathogen, and to identify and map molecular markers linked to this trait. Different segregation populations were used to achieve these objectives, as F2 (160 individuals), BCr (20), and BCs (135), originated from cross the Timor Hybrid UFV 427-15 (CIFC 1343-136), resistant and Catuai Amarelo UFV 2143-236 (IAC 30), susceptible. In the Timor Hybrids populations were detected five gene (SH5 at SH9) that, in isolated way or in association, could confer resistance to the physiologic races of H. vastatrix to the moment identified. The segregation analysis have shown that resistance to coffee rust race II in Timor Hybrid (UFV 427-15, resistant) is conferred by a single dominant gene. The amplification of 852 primers ombinations using bulked segregant analysis and AFLP methodologies, has allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F2 population has shown that they are distributed in both sides of the resistance gene. In one side of the resistance gene, it was found the marker E.CTC/M.TTT405 at 8,69 cM, (LOD =18,91) and E.CGT/M.TGT300 at 28,00 cM (LOD= 4,02), whereas in ther side, the marker E.CCT/M.TTC230 was at 20,50 cM (LOD= 6,15). These markers are the first rust resistance markers identified in Timor Hybrid and can be useful for molecular assisted breeding for rust resistance programs in Brazil.

ASSUNTO(S)

genetic mapping café mapeamento genético coffee fisiologia vegetal coffee rust aflp genetic markers marcadores genéticos ferrugem do cafeeiro aflp

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