Expression of cytokine transcription related to Th17 and T regulatory responses in lesions of chronic periodontitis in type 2 diabetics. / Expressão de citocinas de transcrição relacionados às respostas Th17 e T regulatórias em lesões de periodontite crônica de diabéticos tipo 2.

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

27/02/2012

RESUMO

Although diabetes mellitus (DM) is a well-recognized risk factor for periodontitis, the cellular and molecular mechanisms that link both diseases were not completely elucidated. To date, few studies have evaluated the role of T helper (Th) 17 and T regulatory (Treg) cells in diabetic subjects with chronic periodontitis (CP). Therefore, the aim of this study was to evaluate, using quantitative PCR, the expression of genes related to Th17 and Treg responses [(IL)-17, IL- 6, IL-23, IL-10, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, transcription factor forkhead box p3 (FOXP3) and transcription factor orphan nuclear receptor C2 (RORC2)] in sites with advanced CP in type 2 diabetic subjects with good (glycated hemoglobin [HbA1c] ≤ 8%) and poor (HbA1c >8%) glycemic control. Gingival biopsies were harvested from systemically healthy non-periodontitis subjects (n=15; group 1), systemically healthy subjects with CP (n=15; group 2), better-controlled (HbA1c ≤ 8%) diabetic subjects with and CP (n=15; group 3) and, poorly-controlled (HbA1c >8%) diabetic subjects with and CP (n=15; group 4). The levels of mRNA of interleukin IL-17, IL-6, IL-23, IL-10, TNF-α, TGF-β, FOXP3, RORC2 were evaluated by quantitative PCR. All CP groups presented higher levels of mRNA of IL-17 and a higher frequency of IL-17 mRNA-positive biopsies when compared to group 1 (p<0.05). There was a higher frequency of detection of mRNA of RORC2 in the biopsies from both diabetic groups, when compared to groups 1 and 2 (p<0.05). There were no differences among groups for the frequency and levels of IL-6, IL-23, IL-10, TNF-α, TGF-β and FOXP3 (p>0.05). In conclusion, advanced periodontitis sites in type 2 diabetic subjects, independent of glycemic status, did not present significant differences in the expression of genes related to Th17 and Treg responses, when compared to non-diabetic subjects.

ASSUNTO(S)

diabetes melito periodontite crônica expressão gênica citocinas reação em cadeia de polimerase. diabetes mellitus chronic periodontitis gene expression cytokines polymerase chain reaction. odontologia

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