Estudo de proteinases e citocinas na resposta granulomatosa em camundongos infectados com Paracoccidioides brasiliensis. / Study on proteinases and cytokines in the granulomatous response of mice infected with Paracoccidioides brasiliensis.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

We studied IFN-g, NO, OPN and MMPs in mice infected with the fungus Paracoccidioides brasiliensis (Pb). IFN-g was found in granulomas (Gr) at 15d and increased in resistant mice (A/J) at 120d. At 15d, macrophages and giant cells (MGCs) were more OPN+ in susceptible mice (B10.A) which had high fungal load and low NO. At 120d, A/J had numerous intensely stained OPN(+) cells and higher NO and lower fungal load than B10.A. MMP9 was found in macrophages, MGCs and Pb of infected mice, which had MMP9 and MMP2 activity. At 15d, KOiNOS had Gr with necrosis, altered Pb and OPN; controls had Gr with many Pb and OPN. At 120d, controls had large Gr with many Pb and OPN expression; KOiNOS had compact Gr with OPN+ cells or residual Gr with weak OPN and decreased fungal load. More OPN levels were detected in KOiNOS. OPN is associated with infection severity at the beginning and with some control at later stages of infection and is involved in Gr development and organization. We show the presence of MMPs and suggest their influence in Gr pattern and in Pb dissemination.

ASSUNTO(S)

paracoccidioides brasiliensis metaloproteinases paracoccidioides brasiliensis cytokines paracoccidioidomicose extracellular matrix granuloma paracoccidioidomycosis granuloma matriz extracelular citocinas metalloproteinases

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