Efeitos indesejaveis do uso de safranina como indicador de potencial eletrico de membrana mitocondrial

AUTOR(ES)
DATA DE PUBLICAÇÃO

1986

RESUMO

This work describes experiments showing that safranine, at the concentrations usually employed as a probe of mitochondrial membrane potential, causes significant undesirable side effects on CA POT. ++ transport by liver mitochondria. The major observations are: (i) safranine potentiates the spontaneous CA POT. ++ release from liver mitochondria induced by phosphate or acetoacetate. This is paralelled by potentiation of the release of state -4 -respiration and of the rate of mitochondrial swelling, indicating a generalized effect of the dye on the mitochondrial membrane; (ii) The efflux of mitochondrial CA POT. ++induced by hidroperoxide is stimulated in the presence of safranina even if membrane stabilizers such as MG POT. ++ and ATP are present. It is concluded that the use of safranine to monitor the changes in membrane potential during CA POT. 2+ transport by mitochondria should be avoided or special care be taken. ...Note: The complete abstract is available with the full electronic digital thesis or dissertations.

ASSUNTO(S)

mitocondria - membranas membranas (biologia)

ACESSO AO ARTIGO

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