EFEITO TOXICOGENÉTICO DO POLIMORFISMO ALA16VAL DO GENE MnSOD EM LEUCÓCITOS EXPOSTOS IN VITRO AO METIL MERCÚRIO / TOXIGENETIC EFFECT OF ALA16VAL MnSOD GENE POLYMORPHISM ON LEUCOCYTES IN VITRO EXPOSED TO METHYL MERCURY

AUTOR(ES)

Thaís Doeler Algarve

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

19/03/2012

RESUMO

The environmental contamination by methyl mercury (MeHg) is a great health public problem in some world regions like Amazonia. The MeHg toxic effects seem to be influenced by environmental and genetic factors. However, there are few studies evaluating the genetic influence on MeHg toxicity in humans. Therefore, the aim of this present study was to evaluate the genetic influence of Ala16Val superoxide dismutase manganese dependent gene polymorphism (Ala16Val-MnSOD) on cytotoxic effects of in vitro human leucocytes exposed to MeHg. Subjects were selected from 100 individuals genotyped to Ala16Val-MnSOD polymorphism (26,47,3 years) with different genotpypes (AA=08, VV=06 and AV=12) to perform the in vitro tests. The reactive oxygen species (ROS) production was measured using 27-dichlorofluorescein diacetate (DCFDA) fluorimetric assay and the cell viability was measured using the MTT assay were performed in leucocyte samples with the same subjects exposed and not exposed to MeHg (2,5μM for 6h). The results showed that AA and VVleucocytes exposed to MeHg did not increase the ROS levels when compared to the cells that were not exposed. However, the AV-leucocyte MeHg exposure increased the ROS levels. The cellular viability comparison among different genotypes exposed to MeHg showed lower AAleucocyte viability when compared to VV-leucocytes, whereas heterozygous cells (AV) presented intermediary values. This occurred probabibly due to the fact of AA-leucocytes present a higher basal H2O2 production than other genotypes. The whole of these results suggests toxicogenetic effects of Ala16Val-SOD polymorphism in human cells exposed to MeHg.

ASSUNTO(S)

toxicidade estresse oxidativo ciencias biologicas metil mercúrio polimorfismo mnsod ala16val oxidative stress toxicogenetic methyl mercury sod2 ala16val polymorphism

Documentos Relacionados

• EFEITO IN VITRO DO POLIMORFISMO ALA16VAL DO GENE DA SUPERÓXIDO DISMUTASE DEPENDENTE DE MANGANÊS NO METABOLISMO OXIDATIVO DE LINFÓCITOS
• Estudo da variante polimórfica 47C>T (Ala-9Val) do gene que codifica para a superóxido dismutase dependente de manganês (MnSOD) em pacientes em condições críticas de saúde
• Dietary carotenoid-rich oil supplementation improves exercise-induced anisocytosis in runners: influences of haptoglobin, MnSOD (Val9Ala), CAT (21A/T) and GPX1 (Pro198Leu) gene polymorphisms in dilutional pseudoanemia ("sports anemia")
• Polymorphisms in the Mn-SOD and EC-SOD Genes and Their Relationship to Diabetic Neuropathy in Type 1 Diabetes Mellitus
• Exercise training inducibility of MnSOD protein expression and activity is retained while reducing prooxidant signaling in the heart of senescent rats