Development and Validation of a Rapid and Reliable RP-HPLC-PDA Method for the Quantification of Six Diterpenes in Copaifera duckei, Copaifera reticulata and Copaifera multijuga Oleoresins
AUTOR(ES)
Carneiro, Luiza J., Bianchi, Thamires C., Silva, Jonas J. M. da, Oliveira, Larissa C., Borges, Carly H. G., Lemes, Danieli C., Bastos, Jairo K., Veneziani, Rodrigo C. S., Ambrósio, Sérgio R.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2018-04
RESUMO
Oleoresins from Copaifera species are extensively used in folk medicine in Brazil, which are employed mainly in the production of cosmetic formulations in Brazil, North America and Europe. Considering the lack of validated analytical methods for the analyses of diterpenes in Copaifera oleoresins, it was developed a validated and reliable reversed-phase high-performance liquid chromatography with photodiode array detection (RP-HPLC-PDA) method for the analysis of six diterpenes, including: (-)-polyalthic acid; (13E)-ent-labda-7,13-dien-15-oic acid; ent-8(17)-labden-15,18-dioic acid; (-)-copalic acid; (-)-3β-acetoxycopalic acid and (-)-3β-hydroxycopalic acid. These compounds were isolated from C. duckei, C. reticulata and C. multijuga oleoresins by chromatographic means. The analytical curves were linear with regression coefficients (r2) between 0.9903 and 0.9999. The limits of detection (LOD) and quantification (LOQ) values were 0.35 to 3.09 µg mL-1 and 1.05 to 9.36 µg mL-1, respectively. The method also displayed good precision and accuracy. The developed analytical method is reliable and a useful tool for the analysis of Copaifera oleoresin and its products.
Documentos Relacionados
- Development and Validation of a Rapid and Reliable HPLC-FLD Method for the Quantification of Ciprofloxacin and Enrofloxacin Residues in Zea mays
- Development, Optimization and Validation of an HPLC-PDA Method for Quantification of Taxifolin in the Bark Extract of Pinus pinaster
- RP-HPLC method development and validation for estimation of rivaroxaban in pharmaceutical dosage forms
- Development and validation of a new and economical stability indicating RP-HPLC method for cefixime trihydrate
- Development and validation of a RP-HPLC method to determine the xanthyletin content in biodegradable polymeric nanoparticles