Detection of Pathogenic Yersinia enterocolitica by a Rapid and Sensitive Duplex PCR Assay
AUTOR(ES)
Wannet, Wim J. B.
FONTE
American Society for Microbiology
RESUMO
A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=88570Documentos Relacionados
- Detection of Pathogenic Yersinia enterocolitica by a Rapid and Sensitive Duplex PCR Assay
- Development of a Fluorogenic 5′ Nuclease PCR Assay for Detection of the ail Gene of Pathogenic Yersinia enterocolitica
- Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
- A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains
- Development of a highly specific assay for rapid identification of pathogenic strains of Yersinia enterocolitica based on PCR amplification of the Yersinia heat-stable enterotoxin gene (yst).