A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains
AUTOR(ES)
Benacer, Douadi, Zain, Siti Nursheena Mohd, Lewis, John W., Khalid, Mohd Khairul Nizam Mohd, Thong, Kwai Lin
FONTE
Rev. Soc. Bras. Med. Trop.
DATA DE PUBLICAÇÃO
2017-03
RESUMO
Abstract INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
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