CARACTERIZAÇÃO MOLECULAR DE LENTIVÍRUS DE PEQUENOS RUMINANTES ISOLADOS NO BRASIL / MOLECULAR CHARACTERIZATION OF Small ruminant lentiviruses ISOLATED IN BRAZIL

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

05/12/2011

RESUMO

CAEV and MVV have been considered genetically distinct but antigenically related as pathogens of goats and sheep, respectively. Moreover, it was demonstrated that these viruses are constantly and easily breaking the barrier between sheep and goats, sheep being infected with CAEV and MVV infecting goats. Like all lentiviruses, the genome of CAEV has a high mutation rate and degree of heterogeneity that may be related to the low fidelity of reverse transcriptase, which lacks proof reading activity of the exonuclease subunit. Genetic analysis of Small Ruminants Lentiviruses (SRLVs) may help to understand the genetics, the proteins and the antigenicity of these viruses, their pathogenesis, epidemiology, phylogenetic relationships and thus their inclusion in the newly created subgroups of SRLVs. It may also be relevant to the development of diagnostic tests specific to the local strains. In this sense, the aim of this work was to isolate circulating viral strains and to study the phylogeny with gag and pol sequences, as well as to implement a genomic library of SRLVs isolated in some Brazilian states. Initially, goats and sheep were screened using the AGID technique, confirmed by Western blotting with subsequent isolation of virus in goat synovial membrane (GSM) from positive samples. Nested-PCR was performed to confirm the presence of the virus and for amplification of regions of interest, followed by sequencing of individual samples. Comparisons were made through alignments of the gag and pol regions of the strains, besides assembling the genomic library of isolated SRLVs. The results were the isolation of circulating virus strains of naturally infected animals at Rio Grande do Norte, Ceará, Piauí, Bahia and Minas Gerais states. The strain isolate, called BrRN-CNPC.G1 were considered the first isolation of small ruminant lentiviruses from naturally infected goat in flock of Rio Grande do Norte, Brazil. The strains demonstrated phenotypically litic, type rapid/high. In the present work, virus strains isolated from Ceará, Minas Gerais and Rio Grande do Norte were amplified and sequenced for gag and pol genes. The genealogy proposed for gag and pol gene was compatible with subtypes B from CAEV. The alignment of amino acid sequences from gag gene was divergent between the strains this study and the standart strain CAEV-Cork. The amino acids were more divergent in sequences of the pol gene.

ASSUNTO(S)

lentivírus filogenia gag pol caev mvv. ciencias biologicas lentiviruses phylogeny gag pol caev mvv

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