CaracterizaÃÃo molecular de espÃcies de Metarhizium e patogenicidade sobre Diatraea saccharalis / Molecular characterization of Metarhizium and pathogenicity on Diatraea saccharalis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Fifteen Metarhizium strains isolated from different areas and hosts were analysed upon genetic characteristics and 7 strains upon the pathogenicity to Diatraea saccharalis. The ITS (Internal Transcrided Spacer) molecular markers of rDNA, Intron splice site primer, RAPD and Microsatelite (SSR-Simple Sequence Repeats) were used to evaluate the genetic diversity among strains. The grouping analysis using the UPGMA method, based on the genetic distances of four molecular markers, confirmed the recognized genetic diversity of the genus Metarhizium. The restriction enzymes HaeIII e MspI, presented genetic diversity among the strains when digesting the amplification products of the rDNA locus ITS1-5.8S-ITS2 with the ITS4 and ITS5 primers, the enzyme DraI did not present restriction sites. The introns of nuclear mRNA group were able to discriminate the Metarhizium strains only by using the EI1 primer. The RAPD and Microsatelite techniques were efficient to demonstrate the diversity among the strains. However, the microsatelite (GACA)4 was more sensitive to detect the intra and interspecific variability among different Metarhizium strains. There was no correlation among groups and geographic areas. The strains 4415, 4400 and 4897 caused higher percentage of Diatraea saccharalis larvae mortality. Moreover, there was no correlation among the grouping generated by molecular techniques and percentage of D. saccharalis larvae mortality

ASSUNTO(S)

intron splice site primer intron splice site primer diatraea saccharalis rapd microsatelite rapd metarhizium microssatÃlite diatraea saccharalis metarhizium micologia regiÃo its-rdna region its-rdna

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