CaracterizaÃÃo fenotÃpica e genotÃpica, sensibilidade a antimicrobianos e detecÃÃo de gene de virulÃncia de cepas clÃnicas e ambientais de Burkholderia pseudomallei. / Genotyping, antimicrobial susceptibility and detection of virulence genes of clinical and environmental strains of Burkholderia pseudomallei isolated in Ceara.

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

04/11/2011

RESUMO

Melioidosis is a serious infectious disease caused by Burkholderia pseudomallei, a Gram negative rod, commonly found in soil and water. The disease is endemic in Southeastern Asia and hyperendemic in Northern Australia. Despite the initiation of empiric therapy, mortality remains at 21% in patients with melioidosis in Australia. In Brazil, it is considered an emerging disease, since April 2003, when it was first diagnosed in Ceara, Northeastern Brazil. In the last eight years, thirteen cases were reported, twelve local cases and one case reported by the Dutch government because of a tourist who died of melioidosis after a visit to Ceara. Considering the occurrence of melioidosis in CearÃ, this work aimed at studying these clinical and environmental strains of Burkholderia pseudomallei isolated from Cearà from 2003 to 2011, focusing on the bacterial and molecular identification; determining the susceptibility profile against five antimicrobial agents (amoxicillin/clavulanate, ceftazidime, imipenem, doxycycline and trimethoprim/sulfamethoxazole); genotyping through Random Amplified Polymorphic DNA (RAPD), detecting the virulence gene Type Three Secretion System (TTSS); and analyzing epidemiological and clinical aspects that characterized the emergence of this disease in Brazil. All 20 strains (10 clinical and 10 environment) from B. pseudomallei were accurately identified by both VITEK2  and sequencing of the 16S DNA, showed to be negative for the assimilation of L-arabinose and were positive results for the detection of the virulence gene TTSS. The minimum inhibitory concentrations (MICs) obtained through microdilution in MÃeller-Hinton broth, showed that all (100%) isolates were sensitive to imipenem, doxycycline and trimethoprim-sulfamethoxazole, however, the susceptibility rate to amoxicillin/clavulanate and ceftazidime was of 80 and 90%, respectively, with no differences between clinical and environmental strains. RAPD-PCR showed a genetic relatedness of 63% among the B. pseudomallei strains from the State of CearÃ, which were grouped in two different clusters. This work will contribute to the knowledge of phenotypic and genotypic characteristics of B. pseudomallei strains isolated in Cearà and the update of epidemiological surveillance of melioidosis cases in the state, also contribute to the awareness of agencies health authority for inclusion of Cearà State as an endemic area for this disease.

ASSUNTO(S)

melioidose burkholderia pseudomallei medicina resistÃncia bacteriana. b. pseudomallei melioidosis antimicrobial susceptibility.

ACESSO AO ARTIGO

Documentos Relacionados