Biochemistry answer of caterpillars of Thyrinteina leucoceraea (Lepidoptera: Geometridae), fed with the serine-proteinases inhibitor benzamidine / Resposta bioquímica de lagartas de Thyrinteina leucoceraea (Lepidoptera: Geometridae), submetidas ao inibidor de serinoproteases benzamidina

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The insects may cause considerable losses to plants, necessitating the control of these pests. However, some insects inhabit plants without causing considerable losses. For example, Thyrinteina leucoceraea, native insect, inhabit guava plants, native Myrtaceae harbor, and dont need to be controled. The Eucalyptus ssp., however, is a novel Myrtaceae host in Brazil, from Austrália, and suffer attacks by T. leucoceraea, that became a severe pest to this plant. Plants can defend themselves and use proteinase inhibitors for defense against insects. The introduction of genes that encode proteinase inhibitors into plants can confer significant insect pest resistance to the transformants, moreover, researches have demonstrated the potential of the proteinase inhibitors in the insect food to reduce its development. Thus, the objectives of this work was to verify the production of proteinase inhibitors by guava plants, host apparently resistent, and eucalyptus plants upon T. leucoceraea attack, and to analyse the biochemistry answer of these caterpillars to the serine-proteinase inhibitor benzamidine, in both hosts, in three concentrations: 0.12, 0.25 and 0.5%, to evaluate its possibility use to control this insect. Eucalyptus plants produced more proteinase inhibitors than guava plants. The good development of T. leucoceraea in eucalyptus plants despite the high concentration of proteinase inhibitors, may be due to the elevation to the enzymes activity into the midgut of the caterpillars. The data suggest that T. leucoceraea developed an adaptation to the proteinase inhhibitor produced by eucalyptus plants, by increasing the serine-proteinase activity. This increase may be due to the ativation of the secundary site of the enzimes. The benzamidine interfered the biochemistry answer of the caterpillars, but didnt reduce its proteinase activity, probably due to the adaptation capacity of these insects to the inhibitor

ASSUNTO(S)

trypsin inhibitors inibidores de protease thyrinteina leucoceraea insect-plant relationships relação inseto-planta proteolytic enzymes thyrinteina leucoceraea ciencias biologicas lepidoptera proteinase inhibitors lepidoptera inibidores de tripsina enzimas proteolíticas

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