AvaliaÃÃo da toxicidade tecidual de Melxi e bromelina por via oral em ratos

AUTOR(ES)

Aurelino CÃndido da Silva

DATA DE PUBLICAÇÃO

2005

RESUMO

ÂCrude bromelain, an aqueous extract from pineapples, has been sold in health food stores as a nutritional supplement for âdigestive aidâ. It contains a mixture of different proteins, carbohydrates and other components. Cystein proteinases in crude bromelain are called bromelains, they are stem bromelain (EC 3.4.22.32), comosain, ananain (EC 3.4.22.31) and fruit bromelain (EC 3.4.22.33). In vitro and in vivo studies declare for bromelain a wide range of therapeutic benefits, such as treatment of inflammatory, blood-coagulation-related and malignant diseases. Recent reports suggest for bromelain a paper in infectious and inflammatory colits. Bromelain and other peptides have been documented in plasma after oral intake in humans and significant levels of enzymatic activity were detected in stools after oral administration in mice. Recently, Melxi  a preparation containing bromelain in bee honey became commercially available. In order to evaluate the toxicity, large dosages of bromelain and Melxi  in oral administration were used in rats. Pathogen-free Wistar female rats aged approximately 8 weeks were used. The animals were distributed in four groups, each comprising 10 animals. The rats received orally by gastric gavage Melxi 20 ml/kg (group A), bromelain[15mg/ml] 20 ml/Kg (groupo B), bromelain[1g/Kg] 20 ml/Kg (group C) and NaCl[0,9%] 20 ml/kg (group D). After 24 hours, the rats were sacrificed, lungs, stomach, liver, spleen and kidneys sections were stained with hematoxylin and eosin for morphological study. No macroscopic or microscopic morphological alterations were observed. These results claim for bromelain and Melxi a paper in oral therapy for a set of clinical applications

ASSUNTO(S)

toxicologia bromelain proteolytic enzymes enzimas proteolÃticas toxicology bioquimica bromelina

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