A instabilidade genômica como fator prognóstico e diagnóstico na progressão de queratose actínica para carcinoma espinocelular humano / Genomic instability as a prognostic and diagnostic factor on the progression of human actinic keratosis, to squamous cell carcinoma

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Genomic instability has been widely used to characterize cancer cells. Genetic alterations in human actinic keratosis (AK) and squamous cell carcinomas (SCC) were investigated by the random amplified polymorphic DNA (RAPD) method, and microsatellite analysis. DNA was obtained from Brazilian patients diagnosed and treated in the School of Medicine of University of Sao Paulo out Clinics Hospital. Eight AKs, 24 SCCs, and 4 BCCs, matched to normal skin tissue and/or leukocytes were studied. Microsatellite patterns were obtained with primers specific to amplify D6S251, D6S252, D9S15, D9S50, D9S52, D9S180, D9S196, D9S280, and D9S287, in search of detection Loss of heterozygosity (LOH) and Microsatellite instability (MSI). The RAPD primers were: OPA-2, OPA-7, OPA-13, OPA-17, OPB-8, OPB-13, OPB-17, and OPB-19. A significant correlation was obtained regarding the progress of AK (1/8) to SCC (5/22) detected with the D6S251 microsatellite. DNA fingerprint obtained with RAPD primers were altered in increasing number of samples, according to their histological degree of differentiation. Similar RAPD patterns were observed in tumor and control in 27% AK, 24% SCC I, 9% SCC II, and zero SCC III. These results suggest microsatellite D6S251 and RAPD method to be potential tools in diagnosis and prognosis of AK and SCC.

ASSUNTO(S)

loss of heterozygosity carcinoma squamous cell perda de heterozigosidade carcinoma de células escamosas instabilidade de microssatélites técnica de amplificação ao acaso de dna polimórfico ceratose keratosis microsatellite instability random amplified polymorphic dna technique.

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