Mostrando 1-12 de 12 artigos, teses e dissertações.
1. Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues
In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was perform
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-02
2. HISTÓRIAS E MEMÓRIAS INSTITUCIONAIS A PARTIR DO ACERVO FOTOGRÁFICO DO CENTRO UNIVERSITÁRIO FRANCISCANO (1955 1980) / INSTITUCIONAL HISTORIES AND MEMORIES FROM THE CENTRO UNIVERSITÁRIO FRANCISCANO PHOTOGRAPHIC COLLECTION (1955 1980)
This paper presents the study about the Centro Universitário Franciscanos stories and memories, which can be caught through the analysis of the photographic collection of the institution. The general objective of this work consists on the treatment according to the criteria archival photographic of the Centro Universitário Franciscano, referring to the per
Publicado em: 2010
3. O SERTÃO DE SANTANA DE PARANAÍBA : UM PERFIL DA SOCIEDADE PASTORIL-ESCRAVISTA NO SUL DO ANTIGO MATO GROSSO (1830 - 1888)
This work arose from the need to contribute to the advancement of studies on the creation pastoral activity as important in the formation of the Brazilian society. The predatory extraction promoted the rapid failure of the El Dorados Brazil (Minas Gerais, Mato Grosso and Goias) and acted in the production process so as to offset the capital used in mining to
Publicado em: 2010
4. Diplomática contemporânea como fundamento metodológico da identificação de tipologia documental em arquivos / Contemporary diplomatics as methodological fundament for the identification of documentary typology in archives
This study presents the archival identification processes, especially about the identification of documents developed by documental typology parameters. The identification is a search procedure on the archival document and its producer entity that uses intelligence to search and records data with adequate instruments. This technique is developed in the begin
Publicado em: 2008
5. CONTROLADORAS ESTRANGEIRAS E CONHECIMENTO ORGANIZACIONAL NAS CONTROLADAS BRASILEIRAS: ESTUDO DE CASO NAS ÁREAS DE VAREJO DE DUAS ORGANIZAÇÕES BANCÁRIAS / FOREIGN ACQUIRERS AND ORGANIZATIONAL KNOWLEDGE IN BRAZILIAN ACQUIRED COMPANIES: A CASE-STUDY IN THE RETAIL AREAS OF TWO BANKS
The aim of this dissertation is the identification, description, and explanation of the process through which cultural, psychological, social, political, and administrative factors that are present in a foreign acquirer interact with the Brazilian acquired company, affecting its organizational knowledge. The latest ten years have witnessed the growing debate
Publicado em: 2005
6. Labelling of cells of the mononuclear phagocyte system in routinely processed archival biopsy specimens with monoclonal antibody EBM/11.
When first reported, EBM/11 reacted with mononuclear phagocyte system cells only in fresh frozen sections, but it has been shown to have similar cellular specificity in routine formalin fixed, paraffin wax embedded tissue. This was achieved by limited proteolysis with protease XIV before immunocytochemical staining. In archival biopsy specimens EBM/11 produc
7. K-ras point mutations in routinely processed tissues: non-radioactive screening by single strand conformational polymorphism analysis.
AIMS--To develop a non-radioactive method to screen routinely fixed, paraffin wax embedded specimens for the occurrence of point mutations; to evaluate the single strand conformational polymorphism (SSCP) analysis technique for the detection of K-ras point mutations as a result of electrophoretic mobility shifts. METHODS--DNA was extracted from archival spec
8. Evaluating the arrayed primer extension resequencing assay of TP53 tumor suppressor gene
Identification of mutations in the tumor suppressor gene TP53 has implications for the molecular epidemiology and for the molecular pathology of human cancer. We have developed and evaluated an arrayed primer extension assay for covering both strands of a region of the coding sequence containing more than 95% of the mutations described so far in TP53. On ave
National Academy of Sciences.
9. Evaluation of 13q14 Status in Multiple Myeloma by Digital Single Nucleotide Polymorphism Technology
Chromosome 13q deletions are common in multiple myeloma and other cancers, demonstrating the importance of this region in tumorigenesis. We used a novel single nucleotide polymorphism (SNP)-based technique, digital SNP (dSNP), to identify loss of heterozygosity (LOH) at chromosome 13q in paraffin-embedded bone marrow biopsies from 22 patients with multiple m
American Society for Investigative Pathology.
10. Identification of novel susceptibility loci for Guam neurodegenerative disease: challenges of genome scans in genetic isolates
Amyotrophic lateral sclerosis/parkinsonism–dementia complex (ALS/PDC) is a fatal neurodegenerative disease found in the Chamorro people of Guam and other Pacific Island populations. The etiology is unknown, although both genetic and environmental factors appear important. To identify loci for ALS/PDC, we conducted both genome-wide linkage and association a
Oxford University Press.
11. Insulin-like growth factor I messenger RNA and protein are expressed in the human lymph node and distinctly confined to subtypes of macrophages, antigen-presenting cells, lymphocytes and endothelial cells
Insulin-like growth factor I (IGF-I) is a potent hormone that stimulates growth and differentiation and inhibits apoptosis in numerous tissues. Preliminary evidence suggests that IGF-I exerts differentiating, mitogenic and restoring activities in the immune system but the sites of synthesis of local IGF-I are unknown. Identification of these sites would allo
Blackwell Science Inc.
12. Identification of a New Candidate Locus for Uric Acid Nephrolithiasis
Renal stone formation is a common multifactorial disorder, of unknown etiology, with an established genetic contribution. Lifetime risk for nephrolithiasis is ∼10% in Western populations, and uric acid stones account for 5%–10% of all stones, depending on climatic, dietary, and ethnic differences. We studied a small, isolated founder population in Sardin
The American Society of Human Genetics.